Genetic analysis and mapping of rice (Oryza sativa L.) male-sterile (OsMS-L) mutant

被引:24
作者
Liu, HS
Chu, HW
Li, H
Wang, HM
Wei, JL
Li, N
Ding, SY
Huang, H
Ma, H
Huang, CF
Luo, D
Yuang, Z
Liu, JH
Zhang, DB [1 ]
机构
[1] Shanghai Jiao Tong Univ, Coll Life Sci & Biotechnol, Shanghai 200240, Peoples R China
[2] Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Plant Physiol & Ecol, Shanghai 200030, Peoples R China
[3] Penn State Univ, Huck Inst Life Sci, Dept Biol, University Pk, PA 16802 USA
[4] Shanghai Acad Agr Sci, Agrobiotech Res Ctr, Shanghai 201106, Peoples R China
来源
CHINESE SCIENCE BULLETIN | 2005年 / 50卷 / 02期
关键词
rice (Oryza sativa L.); mutants; male-sterility; gene mapping; molecular marker;
D O I
10.1360/982004-423
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
A rice male-sterile mutant OsMS-L of japonica cultivar 9522 background, was obtained in M4 population treated with Co-60 gamma-Ray. Genetic analysis indicated that the male-sterile phenotype was controlled by a single recessive gene. Results of tissue section showed that at microspore stage, OsMS-L tapetum was retarded. Then tapetal cells expanded and microspores degenerated. No matured pollens were observed in OsMS-L anther locus. To map OsMS-L locus, an F-2 population was constructed from the cross between the OsMS-L (japonica) and LongTeFu B(indica). Firstly, the OsMS-L locus was roughly mapped between two SSR markers, RM109 and RM7562 on chromosome 2. And then eleven polymorphic markers were developed for further fine fine-mapping. At last the OsMS-L locus was mapped between the two InDel markers, Lhs10 and Lhs6 with genetic distance of 0.4 cM, respectively. The region was delimited to 133 kb. All these results were useful for further cloning and functional analysis of OsMS-L.
引用
收藏
页码:122 / 125
页数:4
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