A copper-metalated, hybrid inorganic-organic polymer as an oxidative nuclease

被引：19

作者：

Chandrasekhar, V ^{[1
]}

Athimoolam, A ^{[1
]}

Krishnan, V ^{[1
]}

Azhakar, R ^{[1
]}

Madhavaiah, C ^{[1
]}

Verma, S ^{[1
]}

机构：

[1] Indian Inst Technol, Dept Chem, Kanpur 208016, Uttar Pradesh, India

来源：

EUROPEAN JOURNAL OF INORGANIC CHEMISTRY | 2005年 / 08期

关键词：

chemical nucleases; phosphazenes; pendant polymers; copper complexes; phosphate ester hydrolysis; heterogeneous catalysis;

D O I：

10.1002/ejic.200400727

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

This study describes nucleolytic activity of a copper-metalated, multisite-coordinating, hybrid polymer CPPL-Cu under oxidative conditions. Rapid relaxation of supercoiled plasmid DNA was observed and mechanistic probing by chemical and enzymatic assays revealed involvement of single-oxygen-derived reactive species. The heterogeneous nature of the catalyst allowed a facile recycling of the artificial nuclease. ((c) Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2005).

引用

页码：1482 / 1486

页数：5

共 61 条

[41] Highly specific oxidative damage of double-strand DNA by copper aminoglycosides [J].

Patwardhan, A ;

Cowan, JA .

CHEMICAL COMMUNICATIONS, 2001, (16) :1490-1491

[42] Preparation of the new bis(phenanthroline) ligand "Clip-Phen" and evaluation of the nuclease activity of the corresponding copper complex [J].

Pitie, M ;

Donnadieu, B ;

Meunier, B .

INORGANIC CHEMISTRY, 1998, 37 (14) :3486-3489

[43]

PRUTZ WA, 1994, BIOCHEM J, V302, P373

[44] Catalysis of DNA cleavage by lanthanide complexes with nucleophilic or intercalating ligands and their kinetic characterization [J].

Rammo, J ;

Hettich, R ;

Roigk, A ;

Schneider, HJ .

CHEMICAL COMMUNICATIONS, 1996, (01) :105-107

[45]

Ren R, 2000, INORG CHEM, V39, P5454

[46] Synthesis of a functionalized salen copper complex and its interaction with DNA [J].

Routier, S ;

Bernier, JL ;

Waring, MJ ;

Colson, P ;

Houssier, C ;

Bailly, C .

JOURNAL OF ORGANIC CHEMISTRY, 1996, 61 (07) :2326-2331

[47] SYNTHESIS AND IONIC-CONDUCTIVITY STUDIES OF NEW WATER-INSOLUBLE POLYPHOSPHAZENE POLYMER ELECTROLYTES [J].

SELVARAJ, II ;

CHAKLANOBIS, S ;

CHANDRASEKHAR, V .

JOURNAL OF THE ELECTROCHEMICAL SOCIETY, 1995, 142 (10) :3434-3437

[48] Copolymerization of 2-(4'-vinyl-4-biphenylyloxy) pentachlorocyclotriphosphazene with acrylate and methacrylate monomers [J].

Selvaraj, II ;

Chandrasekhar, V .

POLYMER, 1997, 38 (14) :3617-3623

[49] THERMOTROPIC LIQUID-CRYSTALLINE POLY(ORGANOPHOSPHAZENE) [J].

SINGLER, RE ;

WILLINGHAM, RA ;

NOEL, C ;

FRIEDRICH, C ;

BOSIO, L ;

ATKINS, E .

MACROMOLECULES, 1991, 24 (02) :510-516

[50] Deglycobleomycin: Solid-phase synthesis and DNA cleavage by the resin-bound ligand [J].

Smith, KL ;

Tao, ZF ;

Hashimoto, S ;

Leitheiser, CJ ;

Wu, XH ;

Hecht, SM .

ORGANIC LETTERS, 2002, 4 (07) :1079-1082

← 1 2 3 4 5 6 7 →