Development and Validation of a High-Performance Thin-Layer Chromatography Method for the Determination of Artesunate and Amodiaquine in Tablet Formulations

A simple, cost-effective, precise, accurate, and rapid planar chromatographic method was developed and validated for the separation and determination of amodiaquine and artesunate in tablet formulations. Both compounds were determined using high-performance thin-layer chromatography plates and a mobile phase composed of toluene, acetonitrile, methanol, ammonium acetate, and triethylamine in the ratio 10: 5: 3: 1: 0.5 (% v/v). Amodiaquine was evaluated densitometrically at a detection wavelength of lambda= 345 nm, whereas artesunate was determined fluorimetrically at lambda = 503 nm. The method was linear in the concentration ranges of 0.093-0.280 mu g spot(-1) for artesunate and 0.250-1.250 mu g spot(-1) for amodiaquine, respectively.