In vitro degradation of insoluble lignin in aqueous media by lignin peroxidase and manganese peroxidase

被引：8
|
作者：
Thompson, DN
Hames, BR
Reddy, CA
Grethlein, HE
机构：
[1] Michigan State Univ, Dept Chem Engn, E Lansing, MI 48824 USA
[2] Michigan State Univ, NSF, Ctr Microbial Ecol, E Lansing, MI 48824 USA
[3] Natl Renewable Energy Lab, Biomass Anal Grp, Golden, CO 80401 USA
[4] Michigan State Univ, Dept Microbiol, E Lansing, MI 48824 USA
[5] Michigan Biotechnol Inst, Lansing, MI 48909 USA
来源：
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1998年 / 70-2卷 / 1期
关键词：
lignin peroxidase (LIP); manganese peroxidase (MNP); Phanerochaete chrysosporium;
D O I：
10.1007/BF02920207
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The abilities of lignin peroxidase (LIP) and manganese peroxidase (MNP) from Phanerochaete chrysosporium to degrade an insoluble hardwood Lignin in vitro in aqueous media were tested. Neither LIP nor MNP appreciably changed the mass or lignin content, although both produced small amounts of unique solubilized lignin fragments. Treatment with both LIP and MNP, however, decreased the mass by 11%, decreased the lignin content by 5.1% (4.2% as total weight), and solubilized unique Lignin-derived molecules. These results suggest that LIP and MNP synergistically degrade high molecular weight insoluble lignin, but singly, neither enzyme is sufficient to effect lignin degradation.
引用
收藏
页码：967 / 982
页数：16
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