PIEZO2 mediates ultrasonic hearing via cochlear outer hair cells in mice

被引：28

作者：

Li, Jie ^{[1
,2
]}

Liu, Shuang ^{[1
,2
]}

Song, Chenmeng ^{[1
,2
]}

Hu, Qun ^{[1
,2
]}

Zhao, Zhikai ^{[1
,2
]}

Deng, Tuantuan ^{[2
,3
,4
]}

Wang, Yi ^{[1
,2
]}

Zhu, Tong ^{[1
,2
]}

Zou, Linzhi ^{[1
,2
]}

Wang, Shufeng ^{[1
,2
]}

Chen, Jiaofeng ^{[1
,2
]}

Liu, Lian ^{[1
,2
]}

Hou, Hanqing ^{[2
]}

Yuan, Kexin ^{[1
,5
]}

Zheng, Hairong ^{[6
]}

Liu, Zhiyong ^{[7
]}

Chen, Xiaowei ^{[8
,9
,10
]}

Sun, Wenzhi ^{[11
,12
]}

Xiao, Bailong ^{[2
]}

Xiong, Wei ^{[1
,2
]}

机构：

[1] Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China

[2] Tsinghua Univ, McGovern Inst Brain Res, IDG Int Data Grp, Beijing 100084, Peoples R China

[3] Tsinghua Univ, Sch Pharmaceut Sci, Beijing 100084, Peoples R China

[4] Tsinghua Univ, Tsinghua Peking Joint Ctr Life Sci, Beijing 100084, Peoples R China

[5] Tsinghua Univ, Sch Med, Dept Biomed Engn, Beijing 100084, Peoples R China

[6] Chinese Acad Sci, Shenzhen Inst Adv Technol, Shenzhen Key Lab MRI, Paul C Lauterbur Res Ctr Biomed Imaging, Shenzhen 440305, Peoples R China

[7] Chinese Acad Sci, Shanghai Inst Biol Sci, Ctr Excellence Brain Sci & Intelligence Technol, Inst Neurosci, Shanghai 200031, Peoples R China

[8] Third Mil Med Univ, Brain Res Ctr, Chongqing 400038, Peoples R China

[9] Third Mil Med Univ, State Key Lab Trauma Burns & Combined Injury Brai, Chongqing 400038, Peoples R China

[10] Chinese Acad Sci, Shanghai Inst Biol Sci, Ctr Excellence Brain Sci & Intelligence Technol, Shanghai 200031, Peoples R China

[11] Chinese Inst Brain Res, Beijing 102206, Peoples R China

[12] Capital Med Univ, Sch Basic Med Sci, Beijing 100069, Peoples R China

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2021年 / 118卷 / 28期

基金：

中国国家自然科学基金;

关键词：

PIEZO2; hearing; ultrasonic vocalization; mechanotransduction; hair cells; MECHANOELECTRICAL TRANSDUCTION; CHANNELS; VOCALIZATIONS; MECHANOTRANSDUCTION; GENERATION; RESPONSES; PRESSURE;

D O I：

10.1073/pnas.2101207118

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Ultrasonic hearing and vocalization are the physiological mechanisms controlling echolocation used in hunting and navigation by microbats and bottleneck dolphins and for social communication by mice and rats. The molecular and cellular basis for ultrasonic hearing is as yet unknown. Here, we show that knockout of the mechanosensitive ion channel PIEZO2 in cochlea disrupts ultrasonic- but not low-frequency hearing in mice, as shown by audiometry and acoustically associative freezing behavior. Deletion of Piezo2 in outer hair cells (OHCs) specifically abolishes associative learning in mice during hearing exposure at ultrasonic frequencies. Ex vivo cochlear Ca2+ imaging has revealed that ultrasonic transduction requires both PIEZO2 and the hair-cell mechanotransduction channel. The present study demonstrates that OHCs serve as effector cells, combining with PIEZO2 as an essential molecule for ultrasonic hearing in mice.

引用

页数：11

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