FlexPlex27-highly multiplexed rapid DNA identification for law enforcement, kinship, and military applications

被引：23

作者：

Grover, Ranjana ^{[1
]}

Jiang, Hua ^{[1
]}

Turingan, Rosemary S. ^{[1
]}

French, Julie L. ^{[1
]}

Tan, Eugene ^{[1
]}

Selden, Richard F. ^{[1
]}

机构：

[1] ANDE, 266 Second Ave, Waltham, MA 02451 USA

来源：

INTERNATIONAL JOURNAL OF LEGAL MEDICINE | 2017年 / 131卷 / 06期

关键词：

RapidDNA identification; CODIS; 20; Kinship; Short tandemrepeat;

D O I：

10.1007/s00414-017-1567-9

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

Rapid DNA identification is the use of a rugged, field-deployable system to generate short tandem repeat (STR) profiles in law enforcement, military, immigration, and homeland security applications. A performance verification study was conducted on the ANDE Rapid DNA identification system using FlexPlex27, a highly multiplexed, 27 locus assay that generates data for the expanded CODIS core loci and all additional STR loci required for international databasing. The assay contains 23 autosomal loci (D1S1656, D2S1338, D2S441, D3S1358, D5S81, D6S1043, D7S820, D8S1179, D10S1248, D12S391, D13S317, D16S539, D18S51, D19S433, D21S11, D22S1045, FGA, CSF1PO, Penta E, TH01, vWA, TPOX, and SE33), three Y-chromosomal loci (DYS391, DYS576, and DYS570), and Amelogenin. Study results demonstrate that the instrument is reliable, reproducible, accurate, robust, and ready for a large scale, comprehensive developmental validation by NDIS-participating laboratories. The additional loci in the FlexPlex assay allow for improved STR profile sharing globally, increase the power of discrimination for identification matches, and improve the effectiveness of kinship analyses.

引用

页码：1489 / 1501

页数：13

共 23 条

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