2D-immunoblotting analysis of Sporothrix schenckii cell wall

被引：25

作者：

Ruiz-Baca, Estela ^{[1
]}

Mora-Montes, Hector M. ^{[2
]}

Lopez-Romero, Everardo ^{[2
]}

Toriello, Conchita ^{[3
]}

Mojica-Marin, Virgilio ^{[1
]}

Urtiz-Estrada, Norma ^{[1
]}

机构：

[1] Univ Juarez Estado Durango, Fac Ciencias Quim, Durango, Mexico

[2] Univ Guanajuato, Div Ciencias Nat & Exactas, Dept Biol, Guanajuato, Mexico

[3] Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Mexico City 04510, DF, Mexico

来源：

MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2011年 / 106卷 / 02期

关键词：

sporotrichosis; Sporothrix schenckii; cell wall; glycoproteins; 2D-immunoblotting analysis; BIOCHEMICAL-CHARACTERIZATION; CANDIDA-ALBICANS; SPOROTRICHOSIS; ANTIBODY; ALPHA-1,2-MANNOSIDASE; IDENTIFICATION; PURIFICATION; EXPRESSION; ANTIGENS;

D O I：

10.1590/S0074-02762011000200021

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

We utilized two-dimensional gel electrophoresis and immunoblotting (2D-immunoblotting) with anti-Sporothrix schenckii antibodies to identify antigenic proteins in cell wall preparations obtained from the mycelial and yeast-like morphologies of the fungus. Results showed that a 70-kDa glycoprotein (Gp70) was the major antigen detected in the cell wall of both morphologies and that a 60-kDa glycoprotein was present only in yeast-like cells. In addition to the Gp70, the wall from filament cells showed four proteins with molecular weights of 48, 55, 66 and 67 kDa, some of which exhibited several isoforms. To our knowledge, this is the first 2D-immunoblotting analysis of the S. schenckii cell wall.

引用

页码：248 / 250

页数：3

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