Enzyme-based amperometric galactose biosensors: a review

被引：46

作者：

Kanyong, Prosper ^{[1
,2
]}

Krampa, Francis D. ^{[1
,3
]}

Aniweh, Yaw ^{[1
]}

Awandare, Gordon A. ^{[1
,3
]}

机构：

[1] Univ Ghana, WACCBIP, Legon, Accra, Ghana

[2] Univ Ulster, Sch Engn, Jordanstown BT37 0QB, North Ireland

[3] Univ Ghana, Dept Biochem Cell & Mol Biol, Legon, Accra, Ghana

来源：

MICROCHIMICA ACTA | 2017年 / 184卷 / 10期

基金：

英国惠康基金;

关键词：

Galactose oxidase; Electrochemistry; Enzyme immobilization; Modified electrodes; Hydrogen peroxide; Glucose; Redox mediators; Screen-printed electrodes; Disposable sensors; Galactose dehydrogenase; OXIDASE; FILM; POLYPYRROLE; ELECTRODE; GLUCOSE; CARBON; SENSOR; IMMOBILIZATION; CONSTRUCTION; GRAPHENE;

D O I：

10.1007/s00604-017-2465-z

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This review (with 35 references) summarizes the various strategies used in biosensors for galactose, and their analytical performance. A brief comparison of the enzyme immobilization methods employed and the analytical performance characteristics of a range of galactose biosensors are first summarized in tabular form and then described in detail. Selected examples have been included to demonstrate the various applications of these biosensors to real samples. Following an introduction into the field that covers the significance of sensing galactose in various fields, the review covers biosensors based on the use of galactose oxidase, with a discussion of methods for their immobilization (via crosslinking, adsorption, covalent bonding and entrapment). This is followed by a short section on biosensors based on the use of galactose dehydrogenase. The conclusion section summarizes the state of the art and addresses current challenges.

引用

页码：3663 / 3671

页数：9

共 34 条

[1]

BARON AJ, 1994, J BIOL CHEM, V269, P25095

[2] MULTIPURPOSE ELECTRODE WITH DIFFERENT ENZYME-SYSTEMS BOUND TO COLLAGEN FILMS [J].