A Biodegradable and Biocompatible Regular Nanopattern for Large-Scale Selective Cell Growth

A biodegradable substrate with a regular array of nanopillars fabricated by electron-beam lithography and hot embossing is used to address the mechanisms of nanotopographical control of cell behavior. Two different cell lines cultured on the nanopillars show striking differences in cell coverage. These changes are topography-and cell-dependent, and are not mediated by air bubbles trapped on the nanopattern. For the first time, a strong cell-selective effect of the same nanotopography has been clearly demonstrated on a large area; while fibroblast proliferation is inhibited, endothelial cell spreading is visibly enhanced. The reduced fibroblast proliferation indicates that a reduction of available surface area induced by nanotopography might be the main factor affecting cell growth on nanopatterns. The results presented herein pave the way towards the development of permanent vascular replacements, where non-adhesive, inert, surfaces will induce rapid in situ endothelialization to reduce thrombosis and occlusion.