ADD1/SREBP-1c mediates insulin-induced gene expression linked to the MAP kinase pathway

被引：71

作者：

Kotzka, J ^{[1
]}

Müller-Wieland, D ^{[1
]}

Koponen, A ^{[1
]}

Njamen, D ^{[1
]}

Kremer, L ^{[1
]}

Roth, G ^{[1
]}

Munck, M ^{[1
]}

Knebel, B ^{[1
]}

Krone, W ^{[1
]}

机构：

[1] Univ Cologne, Klin & Poliklin Innere Med 2, Ctr Mol Med, Cologne, Germany

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 249卷 / 02期

关键词：

D O I：

10.1006/bbrc.1998.9161

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of this study was to define the role of sterol regulatory element binding protein (SREBP)-1c, the human homologue to ADD1 (adipocyte determination- and differentiation-dependent factor 1), in insulin-induced gene expression. Transfection studies using SREBP-1-deficient cells and a LDL receptor promoter fragment containing the ADD1/SREBP-1c binding side showed that the effects of insulin and PDGF were abolished compared to control cells and completely reconstituted by overexpressing ADD1/SREBP-1c. Overexpression of upstream activators of MAP kinases, like MEKK1 or MEK1, demonstrated that ADD1/SREBP-1c-mediated effects of insulin and PDGF might be linked to the MAP kinase cascade. The recombinant N-terminal domain of ADD1/SREBP-1c was phosphorylated predominantly on serine and slightly on threonine residues by MAP kinases ERK1 and ERK2 in vitro. This was reversible by alkaline phosphatase. We conclude that ADD1/SREBP-1c mediates gene regulatory effects of insulin as well as PDGF and that this signalling is linked to the MAP kinase cascade. (C) 1998 Academic Press.

引用

页码：375 / 379

页数：5

共 17 条

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