Promotion of Met-tRNAiMet binding to ribosomes by yIF2, a bacterial IF2 homolog in yeast

被引：118

作者：

Choi, SK

Lee, JH

Zoll, WL

Merrick, WC

Dever, TE ^{[1
]}

机构：

[1] NICHHD, Lab Eukaryot Gene Regulat, NIH, Bethesda, MD 20892 USA

[2] Case Western Reserve Univ, Dept Biochem, Cleveland, OH 44106 USA

来源：

SCIENCE | 1998年 / 280卷 / 5370期

关键词：

D O I：

10.1126/science.280.5370.1757

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Delivery of the initiator methionine transfer RNA (Met-tRNA(i)(Met)) to the ribosome is a key step in the initiation of protein synthesis. Previous results have indicated that this step is catalyzed by the structurally dissimilar translation factors in prokaryotes and eukaryotes-initiation factor 2 (IF2) and eukaryotic initiation factor 2 (eIF2), respectively. A bacterial IF2 homolog has been identified in both eukaryotes and archaea. By using a combination of molecular genetic and biochemical studies, the Saccharomyces cerevisiae IF2 homolog is shown to function in general translation initiation by promoting Met-tRNA(i)(Met) binding to ribosomes. Thus, the mechanism of protein synthesis in eukaryotes and prokaryotes is more similar than was previously realized.

引用

页码：1757 / 1760

页数：4

共 33 条

[21] Evidence that GCN1 and GCN20, translational regulators of GCN4, function on elongating ribosomes in activation of eIF2 alpha kinase GCN2 [J].