Analysis of microRNA Expression in Liquid-Based Cytology Samples May Be Useful for Primary Lung Cancer Diagnosis

被引:5
作者
Araki, Yusuke [1 ]
Arihiro, Koji [2 ]
Yamaguchi, Kakuhiro [1 ]
Sakamoto, Shinjiro [1 ]
Horimasu, Yasushi [1 ]
Masuda, Takeshi [1 ]
Miyamoto, Shintaro [1 ]
Nakashima, Taku [1 ]
Iwamoto, Hiroshi [1 ]
Fujitaka, Kazunori [1 ]
Hamada, Hironobu [3 ]
Hattori, Noboru [1 ]
机构
[1] Hiroshima Univ, Grad Sch Biomed & Hlth Sci, Dept Mol & Internal Med, Hiroshima, Japan
[2] Hiroshima Univ, Grad Sch Biomed & Hlth Sci, Dept Phys Anal & Therapeut Sci, Hiroshima, Japan
[3] Hiroshima Univ Hosp, Dept Anat Pathol, Hiroshima, Japan
关键词
microRNA; Lung cancer; Cytology; Bronchoscopy; PROLIFERATION; TUMORIGENESIS; BIOMARKERS; ONCOGENE; MIR-31; SERUM; MIRNA;
D O I
10.1093/ajcp/aqaa278
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Objectives: Bronchoscopy is frequently performed for patients suspected of having lung cancer; however, we sometimes fail to make a definitive diagnosis, resulting in additional invasive testing. Many studies indicate that microRNAs (miRs) are abnormally expressed in cancers. We examined the diagnostic value of 4 miRs (miR-21, miR-31, miR-182, and miR-183) extracted from liquid-based cytology (LBC) samples and validated whether they were diagnostically useful. Methods: We collected 18 surgically resected tissue samples and 136 LBC specimens obtained during bronchoscopic examination at Hiroshima University Hospital. We extracted RNA from these samples and compared the expression of 4 miRs by reverse transcription-quantitative polymerase chain reaction. Results: We confirmed that expression of the 4 miRs was significantly higher in cancer tissues than in tumor-adjacent normal tissues. We examined the expression of these miRs in 125 (cancer cases, 83; noncancer cases, 42) of 136 cytologic samples. Expression of all 4 miRs was significantly higher in patients with lung cancer than in those without lung cancer. Among samples judged as benign or indeterminate, levels of these miRs were also significantly higher in patients with lung cancer than in those without lung cancer. Conclusions: The analysis of miR expression in LBC samples might be helpful for primary lung cancer diagnosis.
引用
收藏
页码:644 / 652
页数:9
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