Characterization of the 8-kilodalton antigens of Taenia solium metacestodes and evaluation of their use in an enzyme-linked immunosorbent assay for serodiagnosis

被引:77
作者
Hancock, K [1 ]
Khan, A [1 ]
Williams, FB [1 ]
Yushak, ML [1 ]
Pattabhi, S [1 ]
Noh, J [1 ]
Tsang, VCW [1 ]
机构
[1] Ctr Dis Control & Prevent, Div Parasit Dis, Atlanta, GA 30341 USA
关键词
D O I
10.1128/JCM.41.6.2577-2586.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The Western blot for cysticercosis, which uses lentil lectin purified glycoprotein (LLGP) antigens extracted from the metacestode of Taenia solium, has been the "gold standard" serodiagnostic assay since it was first described in 1989. We report that the diagnostic antigens at 14, 18, and 21 kDa, as well as some larger disulfide-bonded antigens, are actually all members of a very closely related family of proteins, the 8-kDa antigens. The genes for 18 unique, mature proteins have been identified. Nine of these were chemically synthesized and tested in an enzyme-linked immunosorbent assay with a battery of defined serum samples, including 32 cysticercosis-positive serum samples reactive with the 8-kDa antigens of LLGP on Western blotting, 34 serum samples from patients with other parasitic infections, and 15 normal human serum samples. One of the 8-kDa antigens, TsRS1, is 100% sensitive and 100% specific. TsRS1 will be one component of a cocktail of three to four synthetic or recombinant antigens, based on the diagnostic bands of the Western blot, which will be used for the serodiagnosis of cysticercosis.
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收藏
页码:2577 / 2586
页数:10
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共 50 条
[21]   A NOVEL LIPID-BINDING PROTEIN FROM THE CESTODE MONIEZIA-EXPANSA [J].
JANSSEN, D ;
BARRETT, J .
BIOCHEMICAL JOURNAL, 1995, 311 :49-57
[22]   A SIMPLE METHOD FOR DISPLAYING THE HYDROPATHIC CHARACTER OF A PROTEIN [J].
KYTE, J ;
DOOLITTLE, RF .
JOURNAL OF MOLECULAR BIOLOGY, 1982, 157 (01) :105-132
[23]   NEUROCYSTICERCOSIS AS THE MAIN CAUSE OF LATE-ONSET EPILEPSY IN MEXICO [J].
MEDINA, MT ;
ROSAS, E ;
RUBIODONNADIEU, F ;
SOTELO, J .
ARCHIVES OF INTERNAL MEDICINE, 1990, 150 (02) :325-327
[24]   Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites [J].
Nielsen, H ;
Engelbrecht, J ;
Brunak, S ;
vonHeijne, G .
PROTEIN ENGINEERING, 1997, 10 (01) :1-6
[25]   The role of N-linked carbohydrates in the antigenicity of Taenia solium metacestode glycoproteins of 12, 16 and 18 kD [J].
Obregón-Henao, A ;
Gil, DL ;
Gómez, DI ;
Sanzón, F ;
Teale, JM ;
Restrepo, BI .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2001, 114 (02) :209-215
[26]  
Page RDM, 1996, COMPUT APPL BIOSCI, V12, P357
[27]   Neurocysticercosis in persons with epilepsy in Medellin, Colombia [J].
Palacio, LG ;
Jiménez, I ;
Garcia, HH ;
Jiménez, ME ;
Sánchez, JL ;
Noh, J ;
Ahn, L ;
Mora, O ;
Giraldo, M ;
Tsang, VCW .
EPILEPSIA, 1998, 39 (12) :1334-1339
[28]  
*PAN AM HLTH ORG, 1997, PAHO WHO INFORM CONS
[29]   Characterization of GP39-42 and GP24 antigens from Taenia solium cysticerci and of their antigenic GP10 subunit [J].
Plancarte, A ;
Hirota, C ;
Martínez-Ocaña, J ;
Mendoza-Hernández, G ;
Zenteno, E ;
Flisser, A .
PARASITOLOGY RESEARCH, 1999, 85 (8-9) :680-684
[30]   Laboratory diagnosis of human neurocysticercosis:: Double-blind comparison of enzyme-linked immunosorbent assay and electroimmunotransfer blot assay [J].
Proaño-Narvaez, JV ;
Meza-Lucas, A ;
Mata-Ruiz, O ;
García-Jerónimo, RC ;
Correa, D .
JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (06) :2115-2118