From fixed to FRAP: measuring protein mobility and activity in living cells

被引：488

作者：

Reits, EAJ ^{[1
]}

Neefjes, JJ ^{[1
]}

机构：

[1] Netherlands Canc Inst, Div Tumor Biol, NL-1066 CX Amsterdam, Netherlands

来源：

NATURE CELL BIOLOGY | 2001年 / 3卷 / 06期

关键词：

D O I：

10.1038/35078615

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Experiments with fluorescence recovery after photobleaching (FRAP) started 30 years ago to visualize the lateral mobility and dynamics of fluorescent proteins in living cells. Its popularity increased when non-invasive fluorescent tagging became possible with the green fluorescent protein (GFP). Many researchers use GFP to study the localization of fusion proteins in fixed or living cells, but the same fluorescent proteins can also be used to study protein mobility in living cells. Here we review the potential of FRAP to study protein dynamics and activity within a single living cell. These measurements can be made with most standard confocal laser-scanning microscopes equipped with photobleaching protocols.

引用

页码：E145 / E147

页数：3

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