Determination of deoxynivalenol in grain sorghum by chemiluminescence enzyme immunoassay

A chemiluminescence enzyme-linked immunosorbent assay (CLEIA) with enhanced chemiluminescent was developed for the detection of deoxynivalenol (DON) in grain sorghum. Assay conditions, including concentrations of antibody and enzyme conjugate, Na+ (NaCl), organic solvent (methyl alcohol) and pH were investigated. The optimized CLEIA allowed the DON detection in a linear working range of 0.588-26.783 ng mL(-1) with the IC50 value of 3.968ng mL(-1) and a limit of detection (LOD) of 0.311 ng mL(-1). The CLEIA was one time more sensitive than the colorimetric ELISA by using the same antibody and HRP-conjugate. A series of spiked grain sorghum were detected by the method. The recovery rate ranged from 71.68%-96.56%, and the coefficient variation (CV) ranged from 5.93%-13%.