N-Heterocyclic carbenes meet toll-like receptors

被引：16

作者：

Singh, Ishwar ^{[1
]}

Lee, Dianne S. ^{[1
]}

Huang, Shuaishuai ^{[2
]}

Bhattacharjee, Hridaynath ^{[1
]}

Xu, Wei ^{[1
]}

McLeod, Jennifer F. ^{[1
,3
]}

Crudden, Cathleen M. ^{[1
,4
]}

She, Zhe ^{[1
,3
]}

机构：

[1] Queens Univ, Dept Chem, Chernoff Hall, Kingston, ON K7L 3N6, Canada

[2] Chinese Univ Hong Kong Shenzhen, Sch Sci & Engn, Shenzhen 518172, Peoples R China

[3] Queens Univ, Beaty Water Res Ctr, Mitchell Hall, Kingston, ON K7L 3N6, Canada

[4] Nagoya Univ, Inst Transformat Biomol WPI ITbM, Chikusa Ku, Nagoya, Aichi 4648602, Japan

来源：

CHEMICAL COMMUNICATIONS | 2021年 / 57卷 / 68期

基金：

加拿大自然科学与工程研究理事会;

关键词：

GOLD; PROTEINS; LIGANDS; INNATE;

D O I：

10.1039/d1cc03030b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Combining the stability of the N-heterocyclic carbenes (NHCs) and broad-spectrum recognition of toll-like receptor (TLR) proteins, we report new electrochemical biosensors for bacteria detection. Instead of traditional thiol-gold chemistry, newly synthesized NHCs are employed as the linker molecules to immobilize TLR bio-recognition elements on gold electrodes. Our proof-of-concept methodology includes testing the fidelity of TLR-based electrochemical sensors with NHC linkers. The performance of the biosensors is demonstrated using whole-cell bacterial cultures.

引用

页码：8421 / 8424

页数：5

共 21 条

[1] Biosensors for Whole-Cell Bacterial Detection
Ahmed, Asif
Rushworth, Jo V.
Hirst, Natalie A.
Millner, Paul A.
[J]. CLINICAL MICROBIOLOGY REVIEWS, 2014, 27 (03) : 631 - 646
[2] Toll-like receptors: critical proteins linking innate and acquired immunity
Akira, S
Takeda, K
Kaisho, T
[J]. NATURE IMMUNOLOGY, 2001, 2 (08) : 675 - 680
[3] Simple direct formation of self-assembled N-heterocyclic carbene monolayers on gold and their application in biosensing
Crudden, Cathleen M.
Horton, J. Hugh
Narouz, Mina R.
Li, Zhijun
Smith, Christene A.
Munro, Kim
Baddeley, Christopher J.
Larrea, Christian R.
Drevniok, Benedict
Thanabalasingam, Bheeshmon
McLean, Alastair B.
Zenkina, Olena V.
Ebralidze, Iraklii I.
She, Zhe
Kraatz, Heinz-Bernhard
Mosey, Nicholas J.
Saunders, Lisa N.
Yagi, Akiko
[J]. NATURE COMMUNICATIONS, 2016, 7
[4] Crudden CM, 2014, NAT CHEM, V6, P409, DOI [10.1038/NCHEM.1891, 10.1038/nchem.1891]
[5] New trends in the functionalization of metallic gold: from organosulfur ligands to N-heterocyclic carbenes
Engel, Sabrina
Fritz, Eva-Corinna
Ravoo, Bart Jan
[J]. CHEMICAL SOCIETY REVIEWS, 2017, 46 (08) : 2057 - 2075
[6] Maleimide-Modified Gold Nanoparticles (AuNPs): A Versatile Platform for Interfacial Click Reactions Leading to Chemically Modified AuNPs
Gobbo, Pierangelo
Workentin, Mark S.
[J]. SYNLETT, 2016, 27 (13) : 1919 - 1930
[7] A simple method for improving protein solubility and long-term stability
Golovanov, AP
Hautbergue, GM
Wilson, SA
Lian, LY
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (29) : 8933 - 8939
[8] Loading and lake circulation structures recurrent patterns of water quality on the Toronto - Mississauga waterfront of Lake Ontario
Howell, E. T.
Benoit, N.
[J]. JOURNAL OF GREAT LAKES RESEARCH, 2021, 47 (02) : 323 - 342
[9] Maleimide-thiol adducts stabilized through stretching
Huang, Wenmao
Wu, Xin
Gao, Xiang
Yu, Yifei
Lei, Hai
Zhu, Zhenshu
Shi, Yi
Chen, Yulan
Qin, Meng
Wang, Wei
Cao, Yi
[J]. NATURE CHEMISTRY, 2019, 11 (04) : 310 - 319
[10] Toll-like receptor signaling pathways
Kawasaki, Takumi
Kawai, Taro
[J]. FRONTIERS IN IMMUNOLOGY, 2014, 5

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