Adipogenic function of tetranectin mediated by enhancing mitotic clonal expansion via ERK signaling

被引：10

作者：

Go, Seulgi ^{[1
,2
]}

Park, Jihyun ^{[1
,2
]}

Rahman, Safikur ^{[3
]}

Jin, Juno ^{[1
,2
]}

Choi, Inho ^{[1
,2
]}

Kim, Jihoe ^{[1
,2
]}

机构：

[1] Yeungnam Univ, Dept Med Biotechnol, Gyongsan 38541, South Korea

[2] Yeungnam Univ, Res Inst Cell Culture, Gyongsan 38541, South Korea

[3] BR Ambedkar Bihar Univ, Munshi Singh Coll, Dept Bot, Muzaffarpur 845401, Bihar, India

来源：

BMB REPORTS | 2021年 / 54卷 / 07期

基金：

新加坡国家研究基金会;

关键词：

Adipocyte differentiation; ERK; Mitotic clonal expansion; Serum protein; Tetranectin; ADIPOSE CONVERSION; GENE-EXPRESSION; GROWTH-FACTOR; DIFFERENTIATION; ACTIVATION; PROTEIN; IMMUNOREACTIVITY; IDENTIFICATION; FIBROBLASTS; DEPENDS;

D O I：

10.5483/BMBRep.2021.54.7.024

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tetranectin (TN), an adipogenic serum protein, enhances adipocyte differentiation, however, its functional mechanism has yet to be elucidated. In the present study, we investigated the adipogenic function of TN by using medium containing TN-depleted fetal bovine serum (TN-del-FBS) and recombinant mouse TN (mTN). The adipocyte differentiation of 3T3-L1 cells was significantly enhanced by mTN supplementation essentially at differentiation induction, which indicated a potential role of the protein in the early differentiation phase. The adipogenic effect of mTN was more significant with insulin in the differentiation induction cocktail, implicating their close functional relationship. mTN enhanced not only the proliferation of growing cells, but also mitotic clonal expansion (MCE) that is a prerequisite for adipocyte differentiation in the early phase. Consistently, mTN increased the phosphorylation of ERK in the early phase of adipocyte differentiation. Results of this study demonstrate that the adipogenic function of mTN is mediated by enhancing MCE via ERK signaling.

引用

页码：374 / 379

页数：6

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