Flow Injection Determination of Lactate Using Immobilized Lactate Dehydrogenase Enzyme with Tris(2,2′-Bipyridyl)Ruthenium(III) Chemiluminescence Detection

被引：2

作者：

Rishi, Lubna ^{[1
]}

Yaqoob, Mohammad ^{[1
]}

Asghar, Mohammad ^{[1
]}

Nabi, Abdul ^{[1
]}

Munawar, Nusrat ^{[2
]}

机构：

[1] Univ Balochistan, Dept Chem, Quetta, Pakistan

[2] Sardar Bahadur Khan Women Univ, Dept Chem, Quetta, Pakistan

来源：

ANALYTICAL LETTERS | 2016年 / 49卷 / 05期

关键词：

flow injection analysis; immobilized lactate dehydrogenase; reduced nicotinamide adenine dinucleotide; lactate; Tris(2,2 '-bipyridyl)ruthenium(III); Chemiluminescence; LACTIC-ACID; BLOOD LACTATE; FATTY-ACIDS; IN-VITRO; BIOSENSORS; GLUCOSE; SYSTEM; MICRODIALYSIS; URINE; ASSAY;

D O I：

10.1080/00032719.2015.1017764

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A flow-injection procedure is reported for the determination of L-lactate based on its enhancement on tris(2,2 '-bipyridyl)ruthenium(III) chemiluminescence in the presence of reduced nicotinamide adenine dinucleotide produced using immobilized lactate dehydrogenase. The calibration graph was linear between 4 and 50x10(-7)molL(-1) (r(2)=0.9998) with relative standard deviation from 1.6 to 3.0% (n=4). The limit of detection was 1x10(-7)molL(-1) with a sample throughput of 60 per hour. The effect of common organic compounds and metal ions was studied. The immobilized lactate dehydrogenase exhibited good operational activity at 37 degrees C for one week and storage stability at 4 degrees C over a period of 10 weeks. The method was applied to blood serum, and the results were in good agreement with those obtained by a conventional spectrophotometric method with no significant difference at the 95% confidence level.

引用

页码：654 / 664

页数：11

共 44 条

[1] Screen-printed biosensors for the control of wine quality based on lactate and acetaldehyde determination [J].