Protective Role of Decellularized Human Amniotic Membrane from Oxidative Stress-Induced Damage on Retinal Pigment Epithelial Cells

被引：10

作者：

Krishna, Lekshmi ^{[1
,2
]}

Dhamodaran, Kamesh ^{[1
,7
]}

Subramani, Murali ^{[1
]}

Ponnulagu, Murugeswari ^{[1
]}

Jeyabalan, Nallathambi ^{[3
]}

Meka, Sai Rama Krishna ^{[4
]}

Jayadev, Chaitra ^{[5
]}

Shetty, Rohit ^{[6
]}

Chatterjee, Kaushik ^{[4
]}

Khora, Samanta Sekhar ^{[2
]}

Das, Debashish ^{[1
]}

机构：

[1] Narayana Nethralaya Fdn, Stem Cell Res Lab, GROW Labs, 258-A Bommasandra Ind Area, Bangalore 258 A, Karnataka, India

[2] VIT Univ, Sch Biosci & Technol, Vellore, Tamil Nadu, India

[3] Narayana Nethralaya Fdn, Grow Labs, 258-A Bommasandra Ind Area, Bangalore 258 A, Karnataka, India

[4] Indian Inst Sci, Dept Mat Engn, Bangalore, Karnataka, India

[5] Narayana Nethralaya Eye Inst, Dept Vitreo Retinal Serv, 258-A Bommasandra Ind Area, Bangalore, Karnataka, India

[6] Narayana Nethralaya Eye Inst, Dept Cornea & Refract Surg, 258-A Bommasandra Ind Area, Bangalore, Karnataka, India

[7] Univ Houston, Ocular Surface Inst, Coll Optometry, Dept Basic Sci, Houston, TX 77204 USA

来源：

ACS BIOMATERIALS SCIENCE & ENGINEERING | 2019年 / 5卷 / 01期

关键词：

decellularized human amniotic membrane; retinal pigment epithelium; oxidative stress; RPE CELLS; GROWTH-FACTOR; MESENCHYMAL TRANSITION; POTENTIAL APPLICATIONS; MOLECULAR-MECHANISMS; SIGNALING PATHWAYS; PROTEIN EXPRESSION; RCS RATS; TRANSPLANTATION; ADHESION;

D O I：

10.1021/acsbiomaterials.8b00769

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

Oxidative stress is an important cause for several retinal aging diseases. Cell therapy using a decellularized human amniotic membrane (dHAM) as a tissue scaffold for retinal pigment epithelial cells has a potential therapeutic role under such pathological conditions. This is attributed by the anti-inflammatory, antimicrobial, low-immunogenicity aspects of dHAM, apart from harboring a drug reservoir potential. The underlying mechanisms for maintaining the physiological properties of transplanted cells and their survival in a diseased milieu using dHAM has remained unexplored/unanswered. Hence, we investigated the potential role of dHAM in preserving the cellular functions of retinal pigment epithelium in an oxidative stress environment. Adult human retinal pigment epithelial (ARPE-19) cells were cultured on dHAM or tissue culture dishes under hyperoxia. Gene expression, immunofluorescence staining, enzyme-linked immunosorbent assay (ELISA), and scanning electron microscopy (SEM) were performed to assess the levels of reactive oxygen species, proliferation, apoptosis, epithelial-mesenchymal transition, phagocytosis, and secretion of vascular endothelial factors. These results indicate reduced epithelial-mesenchymal transition, generation of reactive oxygen species (p <= 0.0001), and apoptosis (p <= 0.05) in cells cultured on dHAM, compared to those on tissue culture dishes under oxidative stress conditions. Concomitantly, the secretion of the vascular endothelial growth factor was significantly reduced (p <= 0.01) on dHAM. Phagocytic activity was significantly higher (p <= 0.001) in cells cultured on dHAM and were comparable to those cells cultured on tissue culture dishes. SEM images showed a clustered growth pattern on dHAM compared to an elongated morphology when cultured on tissue culture dishes under oxidative stress conditions. These findings demonstrate the utility of dHAM as a scaffold for growing retinal epithelial cells and to maintain their physiological properties in an oxidative stress condition with a potential to develop regenerative medicine strategies to treat degenerative eye diseases.

引用

页码：357 / 372

页数：31

共 80 条

[61] Decellularized Amniotic Membrane Scaffold Compared to Synthetic PLGA and Hybrid Scaffolds Exhibit Superlative Biomechanical Properties for Tissue Engineering Applications [J].