Immunogenicity, Safety, and Anti-Viral Efficacy of a Subunit SARS-CoV-2 Vaccine Candidate in Captive Black-Footed Ferrets (Mustela nigripes) and Their Susceptibility to Viral Challenge

被引:2
作者
Leon, Ariel E. [1 ]
Garelle, Della [2 ]
Hartwig, Airn [3 ]
Falendysz, Elizabeth A. [1 ]
Ip, Hon S. [1 ]
Lankton, Julia S. [1 ]
Tretten, Tyler N. [2 ]
Spraker, Terry R. [4 ]
Bowen, Richard [3 ]
Rocke, Tonie E. [1 ]
机构
[1] US Geol Survey, Natl Wildlife Hlth Ctr, 6006 Schroeder Rd, Madison, WI 53711 USA
[2] US Fish & Wildlife Serv, Natl Black Footed Ferret Conservat Ctr, 19180 North East Frontage Rd, Carr, CO 80612 USA
[3] Colorado State Univ, Dept Biomed Sci, 3107 Rampart Rd, Ft Collins, CO 80523 USA
[4] Colorado State Univ, Dept Microbiol Immunol & Pathol, 2450 Gillette Dr, Ft Collins, CO 80526 USA
来源
VIRUSES-BASEL | 2022年 / 14卷 / 10期
关键词
SARS-CoV-2; black-footed ferrets; mustelids; vaccination; experimental challenge; SARS-COV; INFECTION; DECLINE;
D O I
10.3390/v14102188
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A preliminary vaccination trial against the emergent pathogen, SARS-CoV-2, was completed in captive black-footed ferrets (Mustela nigripes; BFF) to assess safety, immunogenicity, and anti-viral efficacy. Vaccination and boosting of 15 BFF with purified SARS-CoV-2 S1 subunit protein produced a nearly 150-fold increase in mean antibody titers compared to pre-vaccination titers. Serum antibody responses were highest in young animals, but in all vaccinees, antibody response declined rapidly. Anti-viral activity from vaccinated and unvaccinated BFF was determined in vitro, as well as in vivo with a passive serum transfer study in mice. Transgenic mice that received BFF serum transfers and were subsequently challenged with SARS-CoV-2 had lung viral loads that negatively correlated (p < 0.05) with the BFF serum titer received. Lastly, an experimental challenge study in a small group of BFF was completed to test susceptibility to SARS-CoV-2. Despite viral replication and shedding in the upper respiratory tract for up to 7 days post-challenge, no clinical disease was observed in either vaccinated or naive animals. The lack of morbidity or mortality observed indicates SARS-CoV-2 is unlikely to affect wild BFF populations, but infected captive animals pose a potential risk, albeit low, for humans and other animals.
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页数:15
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