Immunogenicity, Safety, and Anti-Viral Efficacy of a Subunit SARS-CoV-2 Vaccine Candidate in Captive Black-Footed Ferrets (Mustela nigripes) and Their Susceptibility to Viral Challenge

被引:2
作者
Leon, Ariel E. [1 ]
Garelle, Della [2 ]
Hartwig, Airn [3 ]
Falendysz, Elizabeth A. [1 ]
Ip, Hon S. [1 ]
Lankton, Julia S. [1 ]
Tretten, Tyler N. [2 ]
Spraker, Terry R. [4 ]
Bowen, Richard [3 ]
Rocke, Tonie E. [1 ]
机构
[1] US Geol Survey, Natl Wildlife Hlth Ctr, 6006 Schroeder Rd, Madison, WI 53711 USA
[2] US Fish & Wildlife Serv, Natl Black Footed Ferret Conservat Ctr, 19180 North East Frontage Rd, Carr, CO 80612 USA
[3] Colorado State Univ, Dept Biomed Sci, 3107 Rampart Rd, Ft Collins, CO 80523 USA
[4] Colorado State Univ, Dept Microbiol Immunol & Pathol, 2450 Gillette Dr, Ft Collins, CO 80526 USA
来源
VIRUSES-BASEL | 2022年 / 14卷 / 10期
关键词
SARS-CoV-2; black-footed ferrets; mustelids; vaccination; experimental challenge; SARS-COV; INFECTION; DECLINE;
D O I
10.3390/v14102188
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A preliminary vaccination trial against the emergent pathogen, SARS-CoV-2, was completed in captive black-footed ferrets (Mustela nigripes; BFF) to assess safety, immunogenicity, and anti-viral efficacy. Vaccination and boosting of 15 BFF with purified SARS-CoV-2 S1 subunit protein produced a nearly 150-fold increase in mean antibody titers compared to pre-vaccination titers. Serum antibody responses were highest in young animals, but in all vaccinees, antibody response declined rapidly. Anti-viral activity from vaccinated and unvaccinated BFF was determined in vitro, as well as in vivo with a passive serum transfer study in mice. Transgenic mice that received BFF serum transfers and were subsequently challenged with SARS-CoV-2 had lung viral loads that negatively correlated (p < 0.05) with the BFF serum titer received. Lastly, an experimental challenge study in a small group of BFF was completed to test susceptibility to SARS-CoV-2. Despite viral replication and shedding in the upper respiratory tract for up to 7 days post-challenge, no clinical disease was observed in either vaccinated or naive animals. The lack of morbidity or mortality observed indicates SARS-CoV-2 is unlikely to affect wild BFF populations, but infected captive animals pose a potential risk, albeit low, for humans and other animals.
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页数:15
相关论文
共 26 条
[1]  
[Anonymous], APHIS ONE HLTH SARS
[2]   SARS-COV-2 INFECTION AND LONGITUDINAL FECAL SCREENING IN MALAYAN TIGERS (PANTHERA TIGRIS JACKSONI), AMUR TIGERS (PANTHERA TIGRIS ALTAICA), AND AFRICAN LIONS (PANTHERA LEO KRUGERI) AT THE BRONX ZOO, NEW YORK, USA [J].
Bartlett, Susan L. ;
Diel, Diego G. ;
Wang, Leyi ;
Zec, Stephanie ;
Laverack, Melissa ;
Martins, Mathias ;
Caserta, Leonardo Cardia ;
Killian, Mary Lea ;
Terio, Karen ;
Olmstead, Colleen ;
Delaney, Martha A. ;
Stokol, Tracy ;
Ivancic, Marina ;
Jenkins-Moore, Melinda ;
Ingerman, Karen ;
Teegan, Taryn ;
McCann, Colleen ;
Thomas, Patrick ;
McAloose, Denise ;
Sykes, John M. ;
Calle, Paul P. .
JOURNAL OF ZOO AND WILDLIFE MEDICINE, 2020, 51 (04) :733-744
[3]  
Bates D., 2017, PACKAGE LME4 LINEAR
[4]   Peridomestic Mammal Susceptibility to Severe Acute Respiratory Syndrome Coronavirus 2 Infection [J].
Bosco-Lauth, Angela M. ;
Root, J. Jeffrey ;
Porter, Stephanie M. ;
Walker, Audrey E. ;
Guilbert, Lauren ;
Hawvermale, Daphne ;
Pepper, Aimee ;
Maison, Rachel M. ;
Hartwig, Airn E. ;
Gordy, Paul ;
Bielefeldt-Ohmann, Helle ;
Bowen, Richard A. .
EMERGING INFECTIOUS DISEASES, 2021, 27 (08) :2073-2080
[5]   The SARS-CoV ferret model in an infection-challenge study [J].
Chu, Yong-Kyu ;
Ali, Georgia D. ;
Jia, Fuli ;
Li, Qianjun ;
Kelvin, David ;
Couch, Ronald C. ;
Harrod, Kevin S. ;
Hutt, Julie A. ;
Cameron, Cheryl ;
Weiss, Susan R. ;
Jonsson, Colleen B. .
VIROLOGY, 2008, 374 (01) :151-163
[6]   The spike protein of SARS-CoV - a target for vaccine and therapeutic development [J].
Du, Lanying ;
He, Yuxian ;
Zhou, Yusen ;
Liu, Shuwen ;
Zheng, Bo-Jian ;
Jiang, Shibo .
NATURE REVIEWS MICROBIOLOGY, 2009, 7 (03) :226-236
[7]   Antibody titres decline 3-month post-vaccination with BNT162b2 [J].
Favresse, Julien ;
Bayart, Jean-Louis ;
Mullier, Francois ;
Elsen, Marc ;
Eucher, Christine ;
Van Eeckhoudt, Sandrine ;
Roy, Tatiana ;
Wieers, Gregoire ;
Laurent, Christine ;
Dogne, Jean-Michel ;
Closset, Melanie ;
Douxfils, Jonathan .
EMERGING MICROBES & INFECTIONS, 2021, 10 (01) :1495-1498
[8]   Mink, SARS-CoV-2, and the Human-Animal Interface [J].
Fenollar, Florence ;
Mediannikov, Oleg ;
Maurin, Max ;
Devaux, Christian ;
Colson, Philippe ;
Levasseur, Anthony ;
Fournier, Pierre-Edouard ;
Raoult, Didier .
FRONTIERS IN MICROBIOLOGY, 2021, 12
[9]  
Fox J., 2022, PACKAGE CAR CO APLL
[10]   Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with Coronavirus Disease, United States [J].
Harcourt, Jennifer ;
Tamin, Azaibi ;
Lu, Xiaoyan ;
Kamili, Shifaq ;
Sakthivel, Senthil K. ;
Murray, Janna ;
Queen, Krista ;
Tao, Ying ;
Paden, Clinton R. ;
Zhang, Jing ;
Li, Yan ;
Uehara, Anna ;
Wang, Haibin ;
Goldsmith, Cynthia ;
Bullock, Hannah A. ;
Wang, Lijuan ;
Whitaker, Brett ;
Lynch, Brian ;
Gautam, Rashi ;
Schindewolf, Craig ;
Lokugamage, Kumari G. ;
Scharton, Dionna ;
Plante, Jessica A. ;
Mirchandani, Divya ;
Widen, Steven G. ;
Narayanan, Krishna ;
Makino, Shinji ;
Ksiazek, Thomas G. ;
Plante, Kenneth S. ;
Weaver, Scott C. ;
Lindstrom, Stephen ;
Tong, Suxiang ;
Menachery, Vineet D. ;
Thornburg, Natalie J. .
EMERGING INFECTIOUS DISEASES, 2020, 26 (06) :1266-1273