Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Analysis of Gram-Positive, Catalase-Negative Cocci Not Belonging to the Streptococcus or Enterococcus Genus and Benefits of Database Extension

被引：55

作者：

Christensen, Jens Jorgen ^{[1
,2
]}

Dargis, Rimtas ^{[1
,2
]}

Hammer, Monja ^{[2
]}

Justesen, Ulrik Stenz ^{[3
]}

Nielsen, Xiaohui C. ^{[1
]}

Kemp, Michael ^{[2
,3
]}

机构：

[1] Slagelse Hosp, Dept Clin Microbiol, Slagelse, Denmark

[2] Statens Serum Inst, Dept Microbiol Surveillance & Res, DK-2300 Copenhagen, Denmark

[3] Odense Univ Hosp, Dept Clin Microbiol, DK-5000 Odense, Denmark

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2012年 / 50卷 / 05期

关键词：

ROUTINE IDENTIFICATION; BACTERIA; MICROBIOLOGY; REVOLUTION;

D O I：

10.1128/JCM.06339-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry with a Bruker Daltonics microflex LT system was applied to 90 well-characterized catalase-negative, Gram-positive cocci not belonging to the streptococci or enterococci. Biotyper version 2.0.43.1 software was used singly or in combination with a database extension generated in this study with 51 collection strains from 16 genera. Most strains were identified by using both databases individually, and some were identified only by applying the combined database. Thus, the methodology is very useful and the generated database extension was helpful.

引用

页码：1787 / 1791

页数：5

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