Validation of vessel size imaging (VSI) in high-grade human gliomas using magnetic resonance imaging, image-guided biopsies, and quantitative immunohistochemistry

被引:33
作者
Chakhoyan, Ararat [1 ,2 ]
Yao, Jingwen [1 ,2 ,3 ]
Leu, Kevin [1 ,2 ]
Pope, Whitney B. [2 ]
Salamon, Noriko [2 ]
Yong, William [4 ]
Lai, Albert [5 ]
Nghiemphu, Phioanh L. [5 ]
Everson, Richard G. [6 ]
Prins, Robert M. [6 ]
Liau, Linda M. [6 ]
Nathanson, David A. [7 ]
Cloughesy, Timothy F. [5 ]
Ellingson, Benjamin M. [1 ,2 ,3 ,8 ]
机构
[1] Univ Calif Los Angeles, David Geffen Sch Med, Ctr Comp Vis & Imaging Biomarkers, BTIL, Los Angeles, CA 90095 USA
[2] Univ Calif Los Angeles, David Geffen Sch Med, Dept Radiol Sci, Los Angeles, CA 90095 USA
[3] Univ Calif Los Angeles, Henry Samueli Sch Engn & Appl Sci, Dept Bioengn, Los Angeles, CA 90095 USA
[4] Univ Calif Los Angeles, David Geffen Sch Med, Dept Pathol & Lab Med, Div Neuropathol, Los Angeles, CA 90095 USA
[5] Univ Calif Los Angeles, Ronald Reagan UCLA Med Ctr, Dept Neurol, Los Angeles, CA USA
[6] Univ Calif Los Angeles, Ronald Reagan UCLA Med Ctr, Dept Neurosurg, Los Angeles, CA USA
[7] David Geffen UCLA Sch Med, Dept Mol & Med Pharmacol, Los Angeles, CA USA
[8] Univ Calif Los Angeles, David Geffen Sch Med, UCLA Neuro Oncol Program, Los Angeles, CA 90095 USA
关键词
CEREBRAL BLOOD-VOLUME; GRADIENT-ECHO; IN-VIVO; MICROVESSEL DENSITY; CONTRAST; PERFUSION; ANGIOGENESIS; MECHANISMS; MICROCIRCULATION; HETEROGENEITY;
D O I
10.1038/s41598-018-37564-w
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
To evaluate the association between a vessel size index (VSIMRI) derived from dynamic susceptibility contrast (DSC) perfusion imaging using a custom spin-and-gradient echo echoplanar imaging (SAGE-EPI) sequence and quantitative estimates of vessel morphometry based on immunohistochemistry from image-guided biopsy samples. The current study evaluated both relative cerebral blood volume (rCBV) and VSIMRI in eleven patients with high-grade glioma (7 WHO grade III and 4 WHO grade IV). Following 26 MRI-guided glioma biopsies in these 11 patients, we evaluated tissue morphometry, including vessel density and average radius, using an automated procedure based on the endothelial cell marker CD31 to highlight tumor vasculature. Measures of rCBV and VSIMRI were then compared to histological measures. We demonstrate good agreement between VSI measured by MRI and histology; VSIMRI = 13.67 mu m and VSIHistology = 12.60 mu m, with slight overestimation of VSIMRI in grade III patients compared to histology. rCBV showed a moderate but significant correlation with vessel density (r = 0.42, p = 0.03), and a correlation was also observed between VSIMRI and VSIHistology (r = 0.49, p = 0.01). The current study supports the hypothesis that vessel size measures using MRI accurately reflect vessel caliber within high-grade gliomas, while traditional measures of rCBV are correlated with vessel density and not vessel caliber.
引用
收藏
页数:8
相关论文
共 44 条
[1]   Methodology of brain perfusion imaging [J].
Barbier, EL ;
Lamalle, L ;
Décorps, M .
JOURNAL OF MAGNETIC RESONANCE IMAGING, 2001, 13 (04) :496-520
[2]   Improved tumor oxygenation and survival in glioblastoma patients who show increased blood perfusion after cediranib and chemoradiation [J].
Batchelor, Tracy T. ;
Gerstner, Elizabeth R. ;
Emblem, Kyrre E. ;
Duda, Dan G. ;
Kalpathy-Cramer, Jayashree ;
Snuderl, Matija ;
Ancukiewicz, Marek ;
Polaskova, Pavlina ;
Pinho, Marco C. ;
Jennings, Dominique ;
Plotkin, Scott R. ;
Chi, Andrew S. ;
Eichler, April F. ;
Dietrich, Jorg ;
Hochberg, Fred H. ;
Lu-Emerson, Christine ;
Iafrate, A. John ;
Ivy, S. Percy ;
Rosen, Bruce R. ;
Loeffler, Jay S. ;
Wen, Patrick Y. ;
Sorensen, A. Greg ;
Jain, Rakesh K. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2013, 110 (47) :19059-19064
[3]   MR CONTRAST DUE TO INTRAVASCULAR MAGNETIC-SUSCEPTIBILITY PERTURBATIONS [J].
BOXERMAN, JL ;
HAMBERG, LM ;
ROSEN, BR ;
WEISSKOFF, RM .
MAGNETIC RESONANCE IN MEDICINE, 1995, 34 (04) :555-566
[4]  
Caraffi S, 2015, HISTOL HISTOPATHOL, V30, P69, DOI 10.14670/HH-30.69
[5]  
Chen WJ, 2015, INT J CLIN EXP MED, V8, P8709
[6]   MR vascular fingerprinting: A new approach to compute cerebral blood volume, mean vessel radius, and oxygenation maps in the human brain [J].
Christen, T. ;
Pannetier, N. A. ;
Ni, W. W. ;
Qiu, D. ;
Moseley, M. E. ;
Schuff, N. ;
Zaharchuk, G. .
NEUROIMAGE, 2014, 89 :262-270
[7]   Angiogenesis in Glioblastoma [J].
Das, Sunit ;
Marsden, Philip A. .
NEW ENGLAND JOURNAL OF MEDICINE, 2013, 369 (16) :1561-1563
[8]   NMR imaging of changes in vascular morphology due to tumor angiogenesis [J].
Dennie, J ;
Mandeville, JB ;
Boxerman, JL ;
Packard, SD ;
Rosen, BR ;
Weisskoff, RM .
MAGNETIC RESONANCE IN MEDICINE, 1998, 40 (06) :793-799
[9]   Correction of Main and Transmit Magnetic Field (B0 and B1) Inhomogeneity Effects in Multicomponent-Driven Equilibrium Single-Pulse Observation of T1 and T2 [J].
Deoni, Sean C. L. .
MAGNETIC RESONANCE IN MEDICINE, 2011, 65 (04) :1021-1035
[10]  
Donahue KM, 2000, MAGNET RESON MED, V43, P845, DOI 10.1002/1522-2594(200006)43:6<845::AID-MRM10>3.0.CO