Enhanced fluorescence detection of miRNA-16 on a photonic crystal

被引：26
作者：
Frascella, F. [1 ]
Ricciardi, S. [1 ]
Pasquardini, L. [2 ]
Potrich, C. [2 ,3 ]
Angelini, A. [1 ]
Chiado, A. [1 ]
Pederzolli, C. [2 ]
De Leo, N. [4 ]
Rivolo, P. [1 ]
Pirri, C. F. [1 ]
Descrovi, E. [1 ]
机构：
[1] Politecn Torino, Dipartimento Sci Appl & Tecnol, I-10129 Turin, Italy
[2] Fdn Bruno Kessler, I-38123 Povo, TN, Italy
[3] CNR Ist Biofis, I-38123 Povo, TN, Italy
[4] Ist Nazl Ric Metrol, Nanofacil Piemonte, I-10135 Turin, Italy
来源：
ANALYST | 2015年 / 140卷 / 16期
关键词：
MICRORNA EXPRESSION; SURFACE; BIOSENSOR; SENSITIVITY; EXTRACTION; EMISSION; WAVES;
D O I：
10.1039/c5an00889a
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
We report a novel sensing method for fluorescence-labelled microRNAs (miRNAs) spotted on an all-dielectric photonic structure. Such a photonic structure provides an enhanced excitation and a directional beaming of the emitted fluorescence, resulting in a significant improvement of the overall signal collected. As a result, the Limit of Detection (LoD) is demonstrated to decrease by a factor of about 50. A compact read-out system allows a wide-field imaging-based detection, with little or no optical alignment issues, which makes this approach particularly interesting for further development for example in microarray-type bioassays.
引用
收藏
页码：5459 / 5463
页数：5
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