Preparation of DNA-Based Biosensor for Electrochemically Identification of Transgenic Soybean

Since the successful transformation of the first transgenic soybean, the variety and planting area of transgenic soybean have increased. Therefore, transgenic soybean identification has become a necessary procedure in gm research. The development of rapid, sensitive, high-throughput and newly automated detection methods has become a hot spot in the detection of transgenic products. In this paper, we modified a layer of a partially reduced graphene oxide film on the surface of an ionic liquid carbon paste electrode by a green, controlled electrochemical reduction method. We fixed the probe on the electrode surface by covalently bonding the remaining oxygen-containing groups on the electrode surface with an end-modified amino group. Then, the hybridization reaction was detected with methylene blue as an indicator after hybridization between the electrode and the target sequence, and the transgenic soybean sequence was detected by differential pulse voltammetry. We also tested PCR products of DNA extracted from actual samples.