Simultaneous determination of malachite green, brilliant green and crystal violet in grass carp tissues by a broad-specificity indirect competitive enzyme-linked immunosorbent assay

被引:70
作者
Shen, Yu-Dong [1 ]
Deng, Xing-Fei [1 ]
Xu, Zhen-Lin [1 ]
Wang, Yu [1 ]
Lei, Hong-Tao [1 ]
Wang, Hong [1 ]
Yang, Jin-Yi [1 ]
Xiao, Zhi-Li [1 ]
Sun, Yuan-Ming [1 ]
机构
[1] S China Agr Univ, Guangdong Prov Key Lab Food Qual & Safety, Guangzhou 510642, Guangdong, Peoples R China
基金
中国国家自然科学基金;
关键词
Broad-specific immunoassay; Malachite green; Brilliant green; Crystal violet; Grass carp tissues; PERFORMANCE LIQUID-CHROMATOGRAPHY; LEUCOMALACHITE GREEN; GENTIAN-VIOLET; HAPTEN DESIGN; WATER; IMMUNOASSAY; ANTIBODIES; FISH; CHLORPYRIFOS; METABOLITES;
D O I
10.1016/j.aca.2011.09.006
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
An immunizing hapten (4-(carboxymethoxy)phenyl)bis(4-(diethylamino)phenyl)methylium for brilliant green (BC), a triphenylmethane dye with a potential illegal use in fish feeding, was synthesized and used to produce polyclonal antibody (PcAb) against BC. Unexpectedly, the obtained PcAb showed high cross-reactivity (CR) to malachite green (MG) and crystal violet (CV) in an indirect competitive enzyme-linked immunosorbent assay (icELISA). After screening against three heterologous coating antigens, the icELISA exhibited good sensitivity and uniform response to BC (IC50 of 1.98 ng mL(-1) and CR of 100%), MG (IC50 of 1.61 ng mL(-1) and CR of 105%) and CV (IC50 of 1.34 ng mL(-1) and CR of 142%) when using (4-(carboxymethoxy)phenyl)bis(4-(dimethylamino)phenyl)methylium as the coating hapten. Therefore, a broad-specificity icELISA for simultaneous determination of BC. MG and CV was developed. The recoveries of single analyte and mixture of three analytes from spiked grass carp tissues were estimated ranging from 74.94% to 110.39%. A statistically significant correlation of results was obtained between the developed icELISA and previously established HPLC approaches with the food-relevant three triphenylmethane dyes concentration range 1.83-200 ng mL(-1) (R-2 = 0.9224), indicating good accuracy of the icELISA and suitability for the broad-specific detection of the three triphenylmethane dyes in grass carp tissues. (C) 2011 Published by Elsevier B.V.
引用
收藏
页码:148 / 154
页数:7
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