Scattering measurement of single particle for highly sensitive homogeneous detection of DNA in serum

被引：6

作者：

Zhu, Liang ^{[1
,2
]}

Li, Guohua ^{[1
,2
]}

He, Yonghong ^{[1
,2
]}

Tan, Hui ^{[3
]}

Sun, Shuqing ^{[1
,2
]}

机构：

[1] Tsinghua Univ, Grad Sch Shenzhen, Shenzhen Key Lab Minimal Invas Med Technol, Inst Opt Imaging & Sensing, Shenzhen 518055, Peoples R China

[2] Tsinghua Univ, Dept Phys, Beijing 100084, Peoples R China

[3] Shenzhen Univ, Affiliated Hosp 1, Shenzhen Key Lab Neurosurg, Shenzhen 518035, Peoples R China

来源：

TALANTA | 2018年 / 178卷

基金：

美国国家科学基金会;

关键词：

DNA assay; Dark field microscopy; Color analysis; Single particle detection; GOLD NANOPARTICLE PROBES; ABSOLUTE QUANTIFICATION; NANORODS; SIZE; ABSORPTION; ASSAY; SHAPE; DIFFERENTIATION; HYBRIDIZATION;

D O I：

10.1016/j.talanta.2017.09.052

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A highly sensitive homogeneous method for DNA detection has been developed. The system relies on two kinds of gold nanorod (AuNR) probes with complementary DNA sequences to the target DNA. In the presence of the target DNA, two kinds of AuNR probes are assembling into dimers or small aggregates. The target-induced AuNR aggregate has higher scattering intensity than that of a single AuNR because of the plasmonic coupling effect. Dark field microscopy was utilized to image the single particle and measure its scattering intensity. We wrote our own Matlab code and used it to extract the scattering signal of all particles. Difference in distribution of scattering intensity between the single AuNR and its aggregate provides a quantitative basis for the detection of target DNA. A linear dynamic range spanning from 0.1 pM to 1 nM and a detection limit of similar to 30 fM were achieved for the detection of DNA in serum sample.

引用

页码：545 / 551

页数：7

共 49 条

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