An ethanol-tolerant recombinant Escherichia coli expressing Zymomonas mobilis pdc and adhB genes for enhanced ethanol production from xylose

被引：27

作者：

Wang, Zhanchao ^{[2
]}

Chen, Ming ^{[1
]}

Xu, Yuquan ^{[1
]}

Li, Shuying ^{[1
]}

Lu, Wei ^{[1
]}

Ping, Shuzhen ^{[1
]}

Zhang, Wei ^{[1
]}

Lin, Min ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China

[2] China Agr Univ, Coll Biol Sci, Beijing 100094, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 04期

关键词：

alcohol dehydrogenase; Escherichia coli; ethanol; pyruvate decarboxylase; xylose;

D O I：

10.1007/s10529-007-9597-x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An ethanol-tolerant mutant, ET1, was isolated by an enrichment method from Escherichia coli JM109. Strains JM109 and ET1 were transformed with expression vector pZY507bc containing Zymomonas mobilis alcohol dehydrogenase II (adhB) and pyruvate decarboxylase (pdc) genes, resulting in an ethanol-sensitive recombinant strain JMbc and an ethanol-tolerant recombinant strain, ET1bc. Alcohol dehydrogenase and pyruvate decarboxylase activities were 24 and 32% lower, respectively, in JMbc than in ET1bc. ET1bc fermented 10% (w/v) xylose to give 39.4 g ethanol/l (77%, theoretical yield), a 1.3-fold increase compared with the ethanol-sensitive strain JMbc.

引用

页码：657 / 663

页数：7

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