Four pairs of alkaloid enantiomers from Isatis indigotica Fortune Ex Land with neuroprotective effects against H2O2-induced SH-SY5Y cell injury

被引：13
作者：
Xi, Yu-Fei [1 ]
Lou, Li-Li [1 ,3 ]
Han, Feng-Ying [1 ]
Liu, Si-Fan [1 ]
Yao, Guo-Dong [1 ]
Lin, Bin [2 ]
Huang, Xiao-Xiao [1 ]
Wang, Xiao-Bo [3 ]
Song, Shao-Jiang [1 ]
机构：
[1] Shenyang Pharmaceut Univ, Sch Tradit Chinese Mat Med, Key Lab Computat Chem Based Nat Antitumor Drug Re, Shenyang 110016, Peoples R China
[2] Shenyang Pharmaceut Univ, Sch Pharmaceut Engn, Shenyang 110016, Peoples R China
[3] Chinese Peoples Liberat Army Logist Support Force, Dalian 116021, Peoples R China
来源：
BIOORGANIC CHEMISTRY | 2020年 / 96卷
基金：
中国博士后科学基金; 中国国家自然科学基金;
关键词：
Isatis indigotica; Enantiomeric alkaloids; Neuroprotective effects; Apoptosis; OXIDATIVE STRESS; ROOT; SKELETON; LEAVES;
D O I：
10.1016/j.bioorg.2020.103650
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In the current study, four pairs of new enantiomeric alkaloids (1a/1b-4a/4b) were obtained from the leaves of Isatis indigotica Fortune Ex Land. Their structures were elucidated through spectroscopic methods and quantum mechanical calculations. Biologically, all isolates were evaluated for their neuroprotective effects against H2O2-induced SH-SY5Y cell injury. As a result, 1a and 1b exhibited enantioselective neuroprotective effects, further Annexin V-FITC/PI analysis showed that apoptosis ratios of 1a and 1b were reduced to 20.93% and 17.87%, respectively.
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页数：8
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