Protopine, a novel microtubule-stabilizing agent, causes mitotic arrest and apoptotic cell death in human hormone-refractory prostate cancer cell lines

被引：58

作者：

Chen, Chun-Han ^{[1
]}

Liao, Cho-Hwa ^{[1
]}

Chang, Ya-Ling ^{[1
]}

Guh, Jih-Hwa ^{[2
]}

Pan, Shiow-Lin ^{[3
]}

Teng, Che-Ming ^{[1
]}

机构：

[1] Natl Taiwan Univ, Coll Med, Inst Pharmacol, Taipei 10051, Taiwan

[2] Natl Taiwan Univ, Coll Med, Sch Pharm, Taipei 10051, Taiwan

[3] Natl Hlth Res Inst, Inst Biotechnol & Pharmaceut Res, Miaoli, Taiwan

来源：

CANCER LETTERS | 2012年 / 315卷 / 01期

关键词：

Protopine; Microtubules; Mitotic arrest; Cdk1; Apoptosis; INHIBITS TUBULIN POLYMERIZATION; N-TERMINAL KINASE; PHOSPHORYLATION; DOCETAXEL; CYCLIN; MITOXANTRONE; PACLITAXEL; PATHWAYS; BCL-2; DESTABILIZATION;

D O I：

10.1016/j.canlet.2011.09.042

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

In this study, we investigated the anticancer effect of protopine on human hormone-refractory prostate cancer (HRPC) cells. Protopine exhibited an anti-proliferative effect by induction of tubulin polymerization and mitotic arrest, which ultimately led to apoptotic cell death. The data suggest that protopine increased the activity of cyclin-dependent kinase 1 (Cdk1)/cyclin B1 complex and that contributed to cell apoptosis by modulating mitochondria-mediated signaling pathways, such as Bcl-2 phosphorylation and Mcl-1 down-regulation. In conclusion, the data suggest that protopine is a novel microtubule stabilizer with anticancer activity in HRPC cells through apoptotic pathway by modulating Cdk1 activity and BcI-2 family of proteins. (C) 2011 Elsevier Ireland Ltd. All rights reserved.

引用

页码：1 / 11

页数：11

共 42 条

[1] Cyclin-dependent kinase-1: linking apoptosis to cell cycle and mitotic catastrophe [J].

Castedo, M ;

Perfettini, JL ;

Roumier, T ;

Kroemer, G .

CELL DEATH AND DIFFERENTIATION, 2002, 9 (12) :1287-1293

[2] 2-Phenyl-5-(pyrrolidin-1-yl)-1-(3,4,5-trimethoxybenzyl)-1H-benzimidazole, a benzimidazole derivative, inhibits growth of human prostate cancer cells by affecting tubulin and c-Jun N-terminal kinase [J].

Chang, Wei-Ling ;

Chang, Chih-Shiang ;

Chiang, Po-Cheng ;

Ho, Yunn-Fang ;

Liu, Ju-Fang ;

Chang, Kai-Wei ;

Guh, Jih-Hwa .

BRITISH JOURNAL OF PHARMACOLOGY, 2010, 160 (07) :1677-1689

[3] Quinolone analogue inhibits tubulin polymerization and induces apoptosis via Cdk1-involved signaling pathways [J].

Chen, Ying-Cheng ;

Lu, Pin-Hsuan ;

Pan, Shiou-Lin ;

Teng, Che-Ming ;

Kuo, Sheng-Chu ;

Lin, Tsung-Ping ;

Ho, Yunn-Fang ;

Huang, Yu-Chun ;

Guh, Jih-Hwa .

BIOCHEMICAL PHARMACOLOGY, 2007, 74 (01) :10-19

[4] Novel role for JNK as a stress-activated Bcl2 kinase [J].

Deng, XM ;

Xiao, L ;

Lang, WH ;

Gao, FQ ;

Ruvolo, P ;

May, WS .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (26) :23681-23688

[5] Myeloid cell leukemia 1 is phosphorylated through two distinct pathways, one associated with extracellular signal-regulated kinase activation and the other with G2/M accumulation or protein phosphatase 1/2A inhibition [J].

Domina, AM ;

Smith, JH ;

Craig, RW .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (28) :21688-21694

[6] CDC2 PHOSPHORYLATION IS REQUIRED FOR ITS INTERACTION WITH CYCLIN [J].

DUCOMMUN, B ;

BRAMBILLA, P ;

FELIX, MA ;

FRANZA, BR ;

KARSENTI, E ;

DRAETTA, G .

EMBO JOURNAL, 1991, 10 (11) :3311-3319

[7]

Ebneth A, 1999, CELL MOTIL CYTOSKEL, V44, P209, DOI 10.1002/(SICI)1097-0169(199911)44:3<209::AID-CM6>3.0.CO

[8]

2-4

[9] Transport and cytotoxicity of paclitaxel, docetaxel, and novel taxanes in human breast cancer cells [J].

Ehrlichova, M ;

Vaclavikova, R ;

Ojima, I ;

Pepe, A ;

Kuznetsova, LV ;

Chen, J ;

Truksa, J ;

Kovar, J ;

Gut, I .

NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 2005, 372 (01) :95-105

[10] Endoplasmic reticulum stress and apoptosis [J].

Faitova, Jitka ;

Krekac, Daniel ;

Hrstka, Roman ;

Vojtesek, Borivoj .

CELLULAR & MOLECULAR BIOLOGY LETTERS, 2006, 11 (04) :488-505

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