Combining Dietary Intervention with Metformin Treatment Enhances Non-Alcoholic Steatohepatitis Remission in Mice Fed a High-Fat High-Sucrose Diet

被引:4
作者
Baiges-Gaya, Gerard [1 ,2 ]
Rodriguez-Tomas, Elisabet [1 ,2 ]
Castane, Helena [1 ,2 ]
Jimenez-Franco, Andrea [1 ]
Amigo, Nuria [1 ,3 ,4 ]
Camps, Jordi [1 ,2 ]
Joven, Jorge [1 ,2 ,5 ]
机构
[1] Rovira & Virgili Univ URV, Dept Med & Surg, Reus 43201, Spain
[2] Hosp Univ St Joan, Unitat Recerca Biomed URB CRB, Inst Invest Santiaria Pere & Virgili IISPV, Reus 43201, Spain
[3] ISCIII, CIBER Diabet & Associated Metab Dis CIBERDEM, Madrid 28029, Spain
[4] Biosfer Teslab, Reus 43201, Spain
[5] Campus Int Excellence Southern Catalonia, Tarragona 43003, Spain
关键词
obesity; NAFLD; liver; adipose tissue; lipidomic; mice; HEPATIC STEATOSIS; INSULIN-BINDING; OBESE; METABOLISM; PHOSPHATIDYLCHOLINE; DEFICIENCY; INDUCTION; AUTOPHAGY; THERAPY;
D O I
10.3390/biom12121787
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH) are serious health concerns for which lifestyle interventions are the only effective first-line treatment. Dietary interventions are effective in body weight reduction, but not in improving insulin sensitivity and hepatic lipid mobilization. Conversely, metformin increases insulin sensitivity and promotes the inhibition of de novo hepatic lipogenesis. In this study, we evaluated the metformin effectiveness in NASH prevention and treatment, when combined with dietary intervention in male mice fed a high-fat high-sucrose diet (HFHSD). Eighty 5-week-old C57BL/6J male mice were fed a chow or HFHSD diet and sacrificed at 20 or 40 weeks. The HFHSD-fed mice developed NASH after 20 weeks. Lipoprotein and lipidomic analyses showed that the changes associated with diet were not prevented by metformin administration. HFHSD-fed mice subject to dietary intervention combined with metformin showed a 19.6% body weight reduction compared to 9.8% in those mice subjected to dietary intervention alone. Lower hepatic steatosis scores were induced. We conclude that metformin should not be considered a preventive option for NAFLD, but it is effective in the treatment of this disorder when combined with dietary intervention.
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页数:17
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共 49 条
[1]   Restricted cafeteria feeding and treadmill exercise improved body composition, metabolic profile and exploratory behavior in obese male rats [J].
Alvarez-Monell, Adam ;
Subias-Gusils, Alex ;
Marine-Casado, Roger ;
Belda, Xavier ;
Gagliano, Humberto ;
Pozo, Oscar J. ;
Boque, Noemi ;
Caimari, Antoni ;
Armario, Antonio ;
Solanas, Montserrat ;
Escorihuela, Rosa M. .
SCIENTIFIC REPORTS, 2022, 12 (01)
[2]  
Anaconda Software Distribution, 2020, AN DOC
[3]   Enhanced Metabolic Flexibility Associated with Elevated Adiponectin Levels [J].
Asterholm, Ingrid Wernstedt ;
Scherer, Philipp E. .
AMERICAN JOURNAL OF PATHOLOGY, 2010, 176 (03) :1364-1376
[4]   Adipose Tissue-Liver Cross Talk in the Control of Whole-Body Metabolism: Implications in Nonalcoholic Fatty Liver Disease [J].
Azzu, Vian ;
Vacca, Michele ;
Virtue, Samuel ;
Allison, Michael E. D. ;
Vidal-Puig, Antonio .
GASTROENTEROLOGY, 2020, 158 (07) :1899-1912
[5]   Hepatic metabolic adaptation and adipose tissue expansion are altered in mice with steatohepatitis induced by high-fat high sucrose diet [J].
Baiges-Gaya, Gerard ;
Fernandez-Arroyo, Salvador ;
Luciano-Mateo, Fedra ;
Cabre, Noemi ;
Rodriguez-Tomas, Elisabet ;
Hernandez-Aguilera, Anna ;
Castane, Helena ;
Romeu, Marta ;
Nogues, Maria-Rosa ;
Camps, Jordi ;
Joven, Jorge .
JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 2021, 89
[6]   Altered lipid metabolism in the aging kidney identified by three layered omic analysis [J].
Braun, Fabian ;
Rinschen, Markus M. ;
Bartels, Valerie ;
Frommolt, Peter ;
Habermann, Bianca ;
Hoeijmakers, Jan H. J. ;
Schumacher, Bojrn ;
Dolle, Martijn E. T. ;
Mueller, Roman-Ulrich ;
Benzing, Thomas ;
Schermer, Bernhard ;
Kurschat, Christine E. .
AGING-US, 2016, 8 (03) :441-457
[7]   Laparoscopic sleeve gastrectomy alters 1H-NMR-measured lipoprotein and glycoprotein profile in patients with severe obesity and nonalcoholic fatty liver disease [J].
Cabre, Noemi ;
Gil, Miriam ;
Amigo, Nuria ;
Luciano-Mateo, Fedra ;
Baiges-Gaya, Gerard ;
Fernandez-Arroyo, Salvador ;
Rodriguez-Tomas, Elisabet ;
Hernandez-Aguilera, Anna ;
Castane, Helena ;
Paris, Marta ;
Sabench, Fatima ;
Del Castillo, Daniel ;
Camps, Jordi ;
Joven, Jorge .
SCIENTIFIC REPORTS, 2021, 11 (01)
[8]   Intrahepatic fat accumulation and alterations in lipoprotein composition in obese adolescents [J].
Cali, Anna M. G. ;
Zern, Tosca L. ;
Taksali, Sara E. ;
De Oliveira, Ana Mayra ;
Dufour, Sylvie ;
Otvos, James D. ;
Caprio, Sonia .
DIABETES CARE, 2007, 30 (12) :3093-3098
[9]   Aging-related lipidomic changes in mouse serum, kidney, and heart by nanoflow ultrahigh-performance liquid chromatography-tandem mass spectrometry [J].
Eum, Jung Yong ;
Lee, Jong Cheol ;
Yi, Sun Shin ;
Kim, Il Yong ;
Seong, Je Kyung ;
Moon, Myeong Hee .
JOURNAL OF CHROMATOGRAPHY A, 2020, 1618
[10]   Influence of Phospholipid Species on Membrane Fluidity: A Meta-analysis for a Novel Phospholipid Fluidity Index [J].
Fajardo, Val Andrew ;
McMeekin, Lauren ;
LeBlanc, Paul J. .
JOURNAL OF MEMBRANE BIOLOGY, 2011, 244 (02) :97-103