The 3D imaging of mesenchymal stem cells on porous scaffolds using high-contrasted x-ray computed nanotomography

被引：10

作者：

Vojtova, L. ^{[1
]}

Zikmund, T. ^{[1
]}

Pavlinakova, V. ^{[1
]}

Salplachta, J. ^{[1
]}

Kalasova, D. ^{[1
]}

Prosecka, E. ^{[2
]}

Brtnikova, J. ^{[1
]}

Zidek, J. ^{[1
]}

Pavlinak, D. ^{[3
]}

Kaiser, J. ^{[1
]}

机构：

[1] Brno Univ Technol, CEITEC, Purkynova 656-123, Brno 61200, Czech Republic

[2] Inst Expt Med ASCR Vvi, Prague, Czech Republic

[3] Masaryk Univ, Dept Phys Elect, CEPLANT, Brno, Czech Republic

来源：

JOURNAL OF MICROSCOPY | 2019年 / 273卷 / 03期

关键词：

Biopolymeric scaffold; mesenchymal stem cells; SEM/EDX; tissue engineering; X-ray computed nanotomography; ELECTRON-MICROSCOPY; ENGINEERED BONE; SOFT-TISSUE; TOMOGRAPHY; PHASE; FABRICATION; SIZE; CT;

D O I：

10.1111/jmi.12771

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

This study presents an X-ray computed nanotomography (nano-CT) based, high-resolution imaging technique. Thanks to a voxel resolution of 540 nm, this novel technique is suitable for observing the 3D morphology of soft biopolymeric scaffolds seeded with stem cells. A sample of highly porous collagen scaffold seeded with contrasted mesenchymal stem cells (MSC) was investigated by using lab-based nano-CT. The whole volume of the sample was analysed without its destruction. To evaluate the potential of nano-CT, a comparison measurement was done using a standard microscopy technique. Scanning electron microscopy (SEM) combined with energy dispersive X-ray analysis (EDX) established an extension and local accumulation of the contrasting agent - heavy metallic osmium tetroxide. The presented imaging technique is novel as it will help to understand better the behaviour of cells while interacting with three-dimensional biomaterials. This is crucial for both experimental and clinical tissue engineering applications in order to limit the risk of uncontrolled cell growth, and potentially tumour formation.

引用

页码：169 / 177

页数：9

共 53 条

[1] Organization of Extracellular Matrix Fibers Within Polyglycolic Acid-Polylactic Acid Scaffolds Analyzed Using X-Ray Synchrotron-Radiation Phase-Contrast Micro Computed Tomography [J].