Enantioselective separation and determination of the dinotefuran enantiomers in rice, tomato and apple by HPLC

被引:25
作者
Chen, Xiu [1 ]
Dong, Fengshou [1 ]
Liu, Xingang [1 ]
Xu, Jun [1 ]
Li, Jing [1 ]
Li, Yuanbo [1 ]
Wang, Yunhao [2 ]
Zheng, Yongquan [1 ]
机构
[1] Chinese Acad Agr Sci, Inst Plant Protect, Key Lab Pesticide Chem & Applicat, Minist Agr, Beijing 100193, Peoples R China
[2] Minist Agr, China Green Food Dev Ctr, Beijing, Peoples R China
关键词
Chiral pesticides; Dinotefuran; Enantioseparation; Food; Residue; NEONICOTINOID INSECTICIDES; AGRICULTURAL SAMPLES; DEGRADATION; TOXICITY; QUICK; WATER; SOIL;
D O I
10.1002/jssc.201100823
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
An effective chiral analytical method was developed for the resolution and determination of dinotefuran enantiomers in rice, tomato and apple samples. Dinotefuran enantiomers were baseline-separated and determined on a novel chiral column, ChromegaChiral CCA, with n-hexane-ethanol-methanol (85:5:10, v/v/v) as the mobile phase at a flow rate of 1.0 mL/min with UV detection at 270 nm. The resolution of dinotefuran enantiomers was about 1.8. The first eluted enantiomer was (+)-dinotefuran and the second eluted one was (-)-dinotefuran. The effects of mobile-phase composition and column temperature on the enantioseparation were evaluated. The method was validated for linearity, repeatability, accuracy, LOD and LOQ. LOD was 0.15 mg/kg in rice and tomato, 0.05 mg/kg in apple, with an LOQ of 0.5 mg/kg in rice and tomato, 0.2 mg/kg in apple. The average recoveries of the pesticide from all matrices ranged from 75.8 to 92.9% for all fortification levels The precision values associated with the analytical method, expressed as RSD values, were < 16.5% for the pesticide in all matrices. The methodology was successfully applied for the enantioselective analysis of dinotefuran enantiomers in real samples, indicating its efficiency in investigating the environmental stereochemistry of dinotefuran in food matrix.
引用
收藏
页码:200 / 205
页数:6
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