High throughput systems for screening biomembrane interactions on fabricated mercury film electrodes

被引：24

作者：

Coldrick, Zachary ^{[1
]}

Penezic, Abra ^{[2
]}

Gasparovic, Blazenka ^{[2
]}

Steenson, Paul ^{[3
]}

Merrifield, Jon ^{[4
]}

Nelson, Andrew ^{[1
]}

机构：

[1] Univ Leeds, Sch Chem, Ctr Mol Nanosci CMNS, Leeds LS2 9JT, W Yorkshire, England

[2] Ctr Marine & Environm Res, Rudjer Boskovic Inst, Zagreb 10002, Croatia

[3] Univ Leeds, Sch Elect Engn, Leeds LS2 9JT, W Yorkshire, England

[4] Organisense Ltd, Leeds Innovat Ctr, Leeds LS2 9DF, W Yorkshire, England

来源：

JOURNAL OF APPLIED ELECTROCHEMISTRY | 2011年 / 41卷 / 08期

基金：

英国工程与自然科学研究理事会;

关键词：

Flow cell; Mercury film electrode; Wafer based sensing device; Phospholipid monolayer; Adsorption/desorption; INDUCED STRUCTURAL-CHANGES; BILAYER LIPID-MEMBRANE; PHOSPHOLIPID MONOLAYERS; WATER; LIPOSOMES; MODEL; PEPTIDES; PLATINUM; SURFACE; OCTANOL;

D O I：

10.1007/s10800-011-0319-7

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

A sensing system for the rapid on-line screening of lipids and biomembrane active compounds in water samples has been successfully developed. The sensor consists of a flow cell and incorporated wafer-based device with on-chip mercury on platinum (Pt/Hg) working electrode and platinum (Pt) auxiliary and pseudo-reference electrodes. To optimise system performance, the following experiments were carried out: (i) Deposition and removal of phospholipid layers on and from Pt/Hg electrodes respectively, (ii) Effect of electrode size on signal, (iii) Monitoring of different phospholipids deposited in flow cell and, (iv) Detection of phospholipid monolayer interaction with representative compounds. The results showed that: (i) Miniaturisation and ruggedisation of the mercury (Hg)/phospholipid system has been successfully achieved, (ii) Rapid cyclic voltammetry facilitates repetitive dioleoyl phosphatidylcholine (DOPC) monolayer formation on Hg from aqueous DOPC dispersion and, (iii) The device responds selectively to organic compounds injected into electrolyte flow.

引用

页码：939 / 949

页数：11

共 41 条

[21] Electrochemical modeling of electron and proton transfer to ubiquinone-10 in a self-assembled phospholipid monolayer [J].

Moncelli, MR ;

Becucci, L ;

Nelson, A ;

Guidelli, R .

BIOPHYSICAL JOURNAL, 1996, 70 (06) :2716-2726

[22] INITIAL APPLICATIONS OF PHOSPHOLIPID-COATED MERCURY-ELECTRODES TO THE DETERMINATION OF POLYNUCLEAR AROMATIC-HYDROCARBONS AND OTHER ORGANIC MICROPOLLUTANTS IN AQUEOUS SYSTEMS [J].

NELSON, A ;

AUFFRET, N ;

READMAN, J .

ANALYTICA CHIMICA ACTA, 1988, 207 (1-2) :47-57

[23] SUBSTRATE-INDUCED STRUCTURAL-CHANGES IN ELECTRODE-ADSORBED LIPID LAYERS - EXPERIMENTAL-EVIDENCE FROM THE BEHAVIOR OF PHOSPHOLIPID LAYERS ON THE MERCURY WATER INTERFACE [J].

NELSON, A ;

LEERMAKERS, FAM .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1990, 278 (1-2) :73-83

[24] INTERACTION OF HYDROPHOBIC ORGANIC-COMPOUNDS WITH MERCURY ADSORBED DIOLEOYLPHOSPHATIDYLCHOLINE MONOLAYERS [J].

NELSON, A ;

AUFFRET, N ;

BORLAKOGLU, J .

BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1021 (02) :205-216

[25] Conducting gramicidin channel activity in phospholipid monolayers [J].

Nelson, A .

BIOPHYSICAL JOURNAL, 2001, 80 (06) :2694-2703

[26] PHOSPHOLIPID MONOLAYERS AT THE MERCURY WATER INTERFACE [J].

NELSON, A ;

BENTON, A .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1986, 202 (1-2) :253-270

[27] PHOSPHOLIPID MONOLAYERS OF DI-OLEOYL LECITHIN AT THE MERCURY WATER INTERFACE [J].

NELSON, A ;

AUFFRET, N .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1988, 244 (1-2) :99-113

[28] PENETRATION OF MERCURY-ADSORBED PHOSPHOLIPID MONOLAYERS BY POLYNUCLEAR AROMATIC-HYDROCARBONS [J].

NELSON, A .

ANALYTICA CHIMICA ACTA, 1987, 194 :139-149

[29] Electrochemical analysis of a phospholipid phase transition [J].

Nelson, Andrew .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2007, 601 (1-2) :83-93

[30] Electrochemistry of mercury supported phospholipid monolayers and bilayers [J].

Nelson, Andrew .

CURRENT OPINION IN COLLOID & INTERFACE SCIENCE, 2010, 15 (06) :455-466

← 1 2 3 4 5 →