High throughput systems for screening biomembrane interactions on fabricated mercury film electrodes

被引：24

作者：

Coldrick, Zachary ^{[1
]}

Penezic, Abra ^{[2
]}

Gasparovic, Blazenka ^{[2
]}

Steenson, Paul ^{[3
]}

Merrifield, Jon ^{[4
]}

Nelson, Andrew ^{[1
]}

机构：

[1] Univ Leeds, Sch Chem, Ctr Mol Nanosci CMNS, Leeds LS2 9JT, W Yorkshire, England

[2] Ctr Marine & Environm Res, Rudjer Boskovic Inst, Zagreb 10002, Croatia

[3] Univ Leeds, Sch Elect Engn, Leeds LS2 9JT, W Yorkshire, England

[4] Organisense Ltd, Leeds Innovat Ctr, Leeds LS2 9DF, W Yorkshire, England

来源：

JOURNAL OF APPLIED ELECTROCHEMISTRY | 2011年 / 41卷 / 08期

基金：

英国工程与自然科学研究理事会;

关键词：

Flow cell; Mercury film electrode; Wafer based sensing device; Phospholipid monolayer; Adsorption/desorption; INDUCED STRUCTURAL-CHANGES; BILAYER LIPID-MEMBRANE; PHOSPHOLIPID MONOLAYERS; WATER; LIPOSOMES; MODEL; PEPTIDES; PLATINUM; SURFACE; OCTANOL;

D O I：

10.1007/s10800-011-0319-7

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

A sensing system for the rapid on-line screening of lipids and biomembrane active compounds in water samples has been successfully developed. The sensor consists of a flow cell and incorporated wafer-based device with on-chip mercury on platinum (Pt/Hg) working electrode and platinum (Pt) auxiliary and pseudo-reference electrodes. To optimise system performance, the following experiments were carried out: (i) Deposition and removal of phospholipid layers on and from Pt/Hg electrodes respectively, (ii) Effect of electrode size on signal, (iii) Monitoring of different phospholipids deposited in flow cell and, (iv) Detection of phospholipid monolayer interaction with representative compounds. The results showed that: (i) Miniaturisation and ruggedisation of the mercury (Hg)/phospholipid system has been successfully achieved, (ii) Rapid cyclic voltammetry facilitates repetitive dioleoyl phosphatidylcholine (DOPC) monolayer formation on Hg from aqueous DOPC dispersion and, (iii) The device responds selectively to organic compounds injected into electrolyte flow.

引用

页码：939 / 949

页数：11

共 41 条

[1] Continuing electrochemical studies of phospholipid monolayers of dioleoyl phosphatidylcholine at the mercury-electrolyte interface [J].

Bizzotto, D ;

Nelson, A .

LANGMUIR, 1998, 14 (21) :6269-6273

[2] PAST, PRESENT AND FUTURE CONTRIBUTIONS OF MICROELECTRODES TO ANALYTICAL STUDIES EMPLOYING VOLTAMMETRIC DETECTION - A REVIEW [J].

BOND, AM .

ANALYST, 1994, 119 (11) :R1-R21

[3]

Bott A.W., 1995, Current Separations, V14, P64

[4] REVERSED-PHASE THIN-LAYER CHROMATOGRAPHY OF POLYNUCLEAR AROMATIC-HYDROCARBONS AND CHLORINATED BIPHENYLS - RELATIONSHIP WITH HYDROPHOBICITY AS MEASURED BY AQUEOUS SOLUBILITY AND OCTANOL WATER PARTITION-COEFFICIENT [J].

BRUGGEMAN, WA ;

VANDERSTEEN, J ;

HUTZINGER, O .

JOURNAL OF CHROMATOGRAPHY, 1982, 238 (02) :335-346

[5] Phase phenomena in supported lipid films under varying electric potential [J].

Brukhno, Andrey V. ;

Akinshina, Anna ;

Coldrick, Zachary ;

Nelson, Andrew ;

Auer, Stefan .

SOFT MATTER, 2011, 7 (03) :1006-1017

[6] Phospholipid monolayer coated microfabricated electrodes to model the interaction of molecules with biomembranes [J].

Coldrick, Zachary ;

Steenson, Paul ;

Millner, Paul ;

Davies, Matthew ;

Nelson, Andrew .

ELECTROCHIMICA ACTA, 2009, 54 (22) :4954-4962

[7] Tethered-bilayer lipid membranes as a support for membrane-active peptides [J].

Cornell, BA ;

Krishna, G ;

Osman, PD ;

Pace, RD ;

Wieczorek, L .

BIOCHEMICAL SOCIETY TRANSACTIONS, 2001, 29 :613-617

[8] Influence of surface charges on the rupture of black lipid membranes [J].

Diederich, A ;

Bahr, G ;

Winterhalter, M .

PHYSICAL REVIEW E, 1998, 58 (04) :4883-4889

[9] Some bioelectrochemical applications of self-assembled films on mercury [J].

Guidelli, R ;

Becucci, L ;

Dolfi, A ;

Moncelli, MR ;

Buoninsegni, FT .

SOLID STATE IONICS, 2002, 150 (1-2) :13-26

[10] Solid-state reference electrode based on electrodeposited nanoporous platinum for microchip [J].

Han, Ji-Hyung ;

Park, Sejin ;

Boo, Hankil ;

Kim, Hee Chan ;

Nho, Jongmin ;

Chung, Taek Dong .

ELECTROANALYSIS, 2007, 19 (7-8) :786-792

← 1 2 3 4 5 →