Escherichia coli cytochrome c nitrite reductase NrfA

被引：32

作者：

Clarke, Thomas A. ^{[1
]}

Mills, Paul C. ^{[1
]}

Poock, Susie R. ^{[1
]}

Butt, Julea N. ^{[1
]}

Cheesman, Myles R.

Cole, Jeffrey A. ^{[2
]}

Hinton, Jay C. D. ^{[3
]}

Hemmings, Andrew M. ^{[1
]}

Kemp, Gemma

Soderberg, Christopher A. G. ^{[1
]}

Spiro, Stephen ^{[4
]}

Van Wonderen, Jessica

Richardson, David J. ^{[1
]}

机构：

[1] Univ E Anglia, Sch Biol Sci, Ctr Metalloprotein Spect & Biol, Norwich NR4 7TJ, Norfolk, England

[2] Univ Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands, England

[3] AFRC, Inst Food Res, Norwich NR4 7UA, Norfolk, England

[4] Univ Texas Dallas, Dept Mol & Cell Biol, Richardson, TX 75083 USA

来源：

GLOBINS AND OTHER NITRIC OXIDE-REACTIVE PROTEINS, PART B | 2008年 / 437卷

基金：

英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1016/S0076-6879(07)37004-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The periplasmic cytochrome c nitrite reductase (Nrf) system of Escherichia coli utilizes nitrite as a respiratory electron acceptor by reducing it to ammonium. Nitric oxide (NO) is a proposed intermediate in this six-electron reduction and NrfA can use exogenous NO as a substrate. This chapter describes the method used to assay Nrf-catalyzed NO reduction in whole cells of E. coli and the procedures for preparing highly purified NrfA suitable for use in kinetic, spectroscopic, voltammetric, and crystallization studies.

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收藏

页码：63 / 77

页数：15

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