Anaesthetic diethyl ether impairs long-distance electrical and jasmonate signaling in Arabidopsis thaliana

被引:12
作者
Jaksova, Jana [1 ]
Rac, Marek [1 ]
Bokor, Boris [2 ,3 ]
Petrik, Ivan [4 ,5 ]
Novak, Ondrej [4 ,5 ]
Reichelt, Michael [6 ]
Mithoefer, Axel [7 ]
Pavlovic, Andrej [1 ]
机构
[1] Palacky Univ, Fac Sci, Ctr Reg Hana Biotechnol & Agr Res, Dept Biophys, Slechtitelu 27, CZ-78371 Olomouc, Czech Republic
[2] Comenius Univ, Dept Plant Physiol, Fac Nat Sci, Ilkovicova 6,Mlynskei Dolina B2, SK-84215 Bratislava, Slovakia
[3] Comenius Univ, Comenius Univ Sci Pk,Ilkovica 8, SK-84104 Bratislava, Slovakia
[4] Palacky Univ, Fac Sci, Lab Growth Regulators, Slechtitelu 27, CZ-78371 Olomouc, Czech Republic
[5] Czech Acad Sci, Slechtitelu 27, CZ-78371 Olomouc, Czech Republic
[6] Max Planck Inst Chem Ecol, Dept Biochem, Hans Knoll Str 8, D-07745 Jena, Germany
[7] Max Planck Inst Chem Ecol, Res Grp Plant Def Physiol, Hans Knoll Str 8, D-07745 Jena, Germany
关键词
Anaesthesia; Arabidopsis thaliana; Calcium; Electrical signal; Heat wounding; Jasmonic acid; GENE-EXPRESSION; CYTOSOLIC CA2+; SIEVE ELEMENTS; HIGHER-PLANTS; ACID; RESPONSES; PREY; BIOSYNTHESIS; TRANSMISSION; POTENTIALS;
D O I
10.1016/j.plaphy.2021.11.019
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
General volatile anaesthetics (GVA) inhibit electrical signal propagation in animal neurons. Although plants do not have neurons, they generate and propagate electrical signals systemically from a local damaged leaf to neighbouring leaves. This systemic electrical signal propagation is mediated by ligand-gated glutamate receptor-like (GLR) channels. Here, we investigated the effect of GVA diethyl ether on the systemic electrical and further downstream responses in Arabidopsis thaliana. We monitored electrical signals, cytoplasmic Ca2+ level ([Ca2+](cyt)), ultra-weak photon emission, amino acid contents, phytohormone response as well as gene expression in response to heat wounding during diethyl ether anaesthesia. We found complete suppression of electrical and [Ca2+](cyt) signal propagation from damaged leaf to neighbouring systemic leaves upon diethyl ether treatment. Concomitantly, jasmonates (JAs) did not accumulate and expression of JA-responsive genes (AOS, OPR3, JAZ10) was not detected in systemic leaves. However local damaged leaves still showed increased [Ca2+](cyt) and accumulated high level of JAs and JA-inducible transcripts. An exogenously added GLR ligand, L-glutamate, was not able to trigger Ca2+ wave in etherized plants indicating that GLRs are targeted by diethyl ether, but not specifically. The fact that GVA inhibit electrical signal propagation not only in animals but also in plants is intriguing. However, the cellular response is completely blocked only in systemic leaves; the local damaged leaf still senses damaging stimuli.
引用
收藏
页码:311 / 321
页数:11
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