Identification and characterization of novel antioxidant peptides from mackerel (Scomber japonicus) muscle protein hydrolysates

被引：65

作者：

Bashir, Khawaja Muhammad Imran ^{[1
,2
]}

Sohn, Jae Hak ^{[2
,3
]}

Kim, Jin-Soo ^{[4
]}

Choi, Jae-Suk ^{[2
,3
]}

机构：

[1] German Engn Res Ctr Life Sci Technol Med & Enviro, 31 Gwahaksan 1 Ro,60 Bean Gil, Busan 46742, South Korea

[2] Silla Univ, Seafood Res Ctr, IACF, 606 Adv Seafood Proc Complex, Busan 49277, South Korea

[3] Silla Univ, Coll Med & Life Sci, Dept Food Biotechnol, Div Bioind, 140,Baegyang Daero 700 Beon Gil, Busan 46958, South Korea

[4] Gyeongsang Natl Univ, Dept Seafood & Aquaculture Sci, 38 Cheondaegulcchi Gil, Tongyeong Si 53064, Gyeongsangnam D, South Korea

来源：

FOOD CHEMISTRY | 2020年 / 323卷

关键词：

Amino acid sequencing; Bioactive peptide; Enzymatic hydrolysis; Muscle protein hydrolysate; Tandem mass spectrometry; PHYSIOLOGICAL-PROPERTIES; PURIFICATION; SKIN; PRODUCTS;

D O I：

10.1016/j.foodchem.2020.126809

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Antioxidant peptides are commonly used as functional ingredient in the pharmaceutical industries. Here, we characterized the antioxidant peptides from mackerel muscle protein hydrolysates (MPHs). MPHs showing higher bioactivities were separated into seven groups by FPLC. MPH-3 which exhibited significantly higher (p < 0.05) DPPH% scavenging activity (32.12 +/- 3.01%) was fractionated using RP-HPLC to obtain purified fractions A and B, which were further subjected to MALDI-TOF/TOF-MS for mass fingerprinting. Fraction A exhibited the highest (p < 0.05) DPPH% scavenging activity (34.11 +/- 1.52%), and it contained 21 peptides characterized by LC-MS/MS-. Ten peptides were synthesized, and their antioxidant activities were evaluated; one of the peptides, ALSTWTLQLGSTSFSASPM, showed the highest (p < 0.05) DPPH% scavenging activity (36.34 +/- 4.64%) and another peptide, LGTLLFIAIPI, exhibited the highest (p < 0.05) SOD-like activity (28.94 +/- 4.19%). The results of this study indicate that MPHs could serve as a suitable source of antioxidant peptides.

引用

页数：8

共 40 条

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