Production of succinate and polyhydroxyalkanoate from substrate mixture by metabolically engineered Escherichia coli

被引：35

作者：

Kang, Zhen ^{[2
]}

Du, Lili ^{[2
]}

Kang, Junhua ^{[2
]}

Wang, Yang ^{[2
]}

Wang, Qian ^{[1
,2
]}

Liang, Quanfeng ^{[2
]}

Qi, Qingsheng ^{[1
,2
]}

机构：

[1] Shandong Univ, Sch Life Sci, Natl Glycoengn Res Ctr, Jinan 250100, Peoples R China

[2] Shandong Univ, Sch Life Sci, State Key Lab Microbial Technol, Jinan 250100, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2011年 / 102卷 / 11期

基金：

中国国家自然科学基金;

关键词：

Succinate; PHA; Escherichia coli; Metabolic engineering; Biorefinery; PSEUDOMONAS-AERUGINOSA; ANAEROBIC FERMENTATION; REGULATED EXPRESSION; ENZYME-ACTIVITY; ACID PRODUCTION; BIOFUELS; GLYCEROL; BIOREFINERY; STRAINS; FEEDSTOCKS;

D O I：

10.1016/j.biortech.2011.03.070

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The strategic design of this study aimed at producing succinate and polyhydroxyalkanoate (PHA) from substrate mixture of glycerol/glucose and fatty acid in Escherichia coli. To accomplish this, an E. coli KNSP1 strain derived from E. coli LR1110 was constructed by deletions of ptsG, sdhA and pta genes and overexpression of phaC1 from Pseudomonas aeruginosa. Cultivation of E. coli KNSP1 showed that this strain was able to produce 21.07 g/L succinate and 0.54 g/L PHA (5.62 wt.% of cell dry weight) from glycerol and fatty acid mixture. The generated PHA composed of 58.7 mol% 3-hydroxyoctanoate (3HO) and 41.3 mol% 3-hydroxydecanoate (3HD). This strain would be useful for complete utilization of byproducts glycerol and fatty acid of biodiesel production process. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：6600 / 6604

页数：5

共 34 条

[1] VECTORS BEARING A HYBRID TRP-LAC PROMOTER USEFUL FOR REGULATED EXPRESSION OF CLONED GENES IN ESCHERICHIA-COLI [J].

AMANN, E ;

BROSIUS, J ;

PTASHNE, M .

GENE, 1983, 25 (2-3) :167-178

[2] Conversion of Glycerol to Poly(3-Hydroxypropionate) in Recombinant Escherichia coli [J].

Andreessen, Bjoern ;

Lange, Alvin Brian ;

Robenek, Horst ;

Steinbuechel, Alexander .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2010, 76 (02) :622-626

[3] Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels [J].

Atsumi, Shota ;

Hanai, Taizo ;

Liao, James C. .

NATURE, 2008, 451 (7174) :86-U13

[4] Metabolic engineering of Escherichia coli for the production of succinate from glycerol [J].

Blankschien, Matthew D. ;

Clomburg, JamesM. ;

Gonzalez, Ramon .

METABOLIC ENGINEERING, 2010, 12 (05) :409-419

[5] Feedstocks for the future - Biorefinery production of chemicals from renewable carbon [J].

Bozell, Joseph J. .

CLEAN-SOIL AIR WATER, 2008, 36 (08) :641-647

[6] Mutation of the ptsC gene results in increased production of succinate in fermentation of glucose by Escherichia coli [J].

Chatterjee, R ;

Millard, CS ;

Champion, K ;

Clark, DP ;

Donnelly, MI .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (01) :148-154

[7] A microbial polyhydroxyalkanoates (PHA) based bio- and materials industry [J].

Chen, Guo-Qiang .

CHEMICAL SOCIETY REVIEWS, 2009, 38 (08) :2434-2446

[8] Succinic acid production by Actinobacillus succinogenes using hydrolysates of spent yeast cells and corn fiber [J].

Chen, Ke-Quan ;

Li, Jian ;

Ma, Jiang-Feng ;

Jiang, Min ;

Wei, Ping ;

Liu, Zhong-Min ;

Ying, Han-Jie .

BIORESOURCE TECHNOLOGY, 2011, 102 (02) :1704-1708

[9] Green chemistry for the second generation biorefinery - sustainable chemical manufacturing based on biomass [J].

Clark, James H. .

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 2007, 82 (07) :603-609

[10] One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products [J].

Datsenko, KA ;

Wanner, BL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (12) :6640-6645

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