A simple protocol for isolation of fungal DNA

被引：41

作者：

Tendulkar, SR

Gupta, A

Chattoo, BB ^{[1
]}

机构：

[1] Maharaja Sayajirao Univ Baroda, Fac Sci, Dept Microbiol, Vadodara 390002, Gujarat, India

[2] Maharaja Sayajirao Univ Baroda, Fac Sci, Ctr Biotechnol, Vadodara 390002, Gujarat, India

来源：

BIOTECHNOLOGY LETTERS | 2003年 / 25卷 / 22期

关键词：

genomic DNA; Magnaporthe grisea; microwave treatment; PCR; RAPD;

D O I：

10.1023/B:BILE.0000003990.27624.04

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Genomic DNA was isolated from as little as 2 mg dry biomass of Magnaporthe grisea by microwave treatment within 30 s. The quantity of DNA was good enough for PCR analysis and Dot blot hybridization. This technique can be used for various studies, such as DNA fingerprinting to study the population structure of the phytopathogen in different regions, and for a quick screening of M. grisea transformants.

引用

页码：1941 / 1944

页数：4

共 16 条

[1]

ASHKTORAB H, 1992, BIOTECHNIQUES, V13, P198

[2]

BALHADERE PV, 2000, ANN PLANT REV, V4, P1

[3] A RABID METHOD FOR THE ISOLATION OF GENOMIC DNA FROM ASPERGILLUS-FUMIGATUS [J].