A C-HCR assembly of branched DNA nanostructures for amplified uracil-DNA glycosylase assays

被引：38
|
作者：
Wang, Jing [1 ]
Pan, Min [1 ]
Wei, Jie [1 ]
Liu, Xiaoqing [1 ]
Wang, Fuan [1 ]
机构：
[1] Wuhan Univ, Coll Chem & Mol Sci, Minist Educ, Key Lab Analyt Chem Biol & Med, Wuhan 430072, Hubei, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2017年 / 53卷 / 96期
基金：
中国国家自然科学基金;
关键词：
HYBRIDIZATION CHAIN-REACTION; ULTRASENSITIVE DETECTION; AMPLIFICATION STRATEGY; SPATIAL-ORGANIZATION; SUBSTRATE; CIRCUITS; COMPLEX; SIGNAL; CELLS;
D O I：
10.1039/c7cc07057h
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
An autonomous nonenzymatic DNA machine has been successfully engineered based on a two-layered cascaded hybridization chain reaction (C-HCR) circuit, in which the tandem outputs of the upstream HCR-1 unit activate the downstream HCR-2 unit to induce successive repeated hybridizations, generating branched DNA structures and enabling sensitive and selective detection of uracil-DNA glycosylase and its inhibitors.
引用
收藏
页码：12878 / 12881
页数：4
相关论文
共 50 条
  • [31] Crystal Structure of the Vaccinia Virus Uracil-DNA Glycosylase in Complex with DNA
    Burmeister, Wim P.
    Tarbouriech, Nicolas
    Fender, Pascal
    Contesto-Richefeu, Celine
    Peyrefitte, Christophe N.
    Iseni, Frederic
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2015, 290 (29) : 17923 - 17934
  • [32] URACIL-DNA GLYCOSYLASE ACTIVITY IN CHRONIC LYMPHOPROLIFERATIVE DISORDERS
    KOISTINEN, P
    EEROLA, E
    VILPO, JA
    LEUKEMIA RESEARCH, 1986, 10 (11) : 1307 - 1311
  • [33] SECONDARY STRUCTURE OF URACIL-DNA GLYCOSYLASE INHIBITOR PROTEIN
    BALASUBRAMANIAN, S
    BEGER, RD
    BENNETT, SE
    MOSBAUGH, DW
    BOLTON, PH
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (01) : 296 - 303
  • [34] URACIL-DNA GLYCOSYLASE FROM BACILLUS-STEAROTHERMOPHILUS
    KABOEV, OK
    LUCHKINA, LA
    AKHMEDOV, AT
    BEKKER, ML
    FEBS LETTERS, 1981, 132 (02) : 337 - 340
  • [35] URACIL-DNA GLYCOSYLASE OF THERMOPHILIC THERMOTHRIX-THIOPARA
    KABOEV, OK
    LUCHKINA, LA
    KUZIAKINA, TI
    JOURNAL OF BACTERIOLOGY, 1985, 164 (01) : 421 - 424
  • [36] Uracil-DNA glycosylase efficiency is modulated by substrate rigidity
    Poddar, Souvik
    Pratt, Aerial M.
    Orndorff, Paul B.
    van der Vaart, Arjan
    Van Horn, Wade D.
    Levitus, Marcia
    BIOPHYSICAL JOURNAL, 2023, 122 (03) : 149A - 149A
  • [37] Uracil-DNA glycosylase efficiency is modulated by substrate rigidity
    Paul B. Orndorff
    Souvik Poddar
    Aerial M. Owens
    Nikita Kumari
    Bryan T. Ugaz
    Samrat Amin
    Wade D. Van Horn
    Arjan van der Vaart
    Marcia Levitus
    Scientific Reports, 13
  • [38] Complexes of the uracil-DNA glycosylase inhibitor protein, Ugi, with Mycobacterium smegmatis and Mycobacterium tuberculosis uracil-DNA glycosylases
    Acharya, N
    Kumar, P
    Varshney, U
    MICROBIOLOGY-SGM, 2003, 149 : 1647 - 1658
  • [39] Uracil-DNA glycosylase in the extreme thermophile Archaeoglobus fulgidus
    Sandigursky, M
    Franklin, WA
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (25) : 19146 - 19149
  • [40] Multiple uracil-DNA glycosylase activities in Deinococcus radiodurans
    Sandigursky, M
    Sandigursky, S
    Sonati, P
    Daly, MJ
    Franklin, WA
    DNA REPAIR, 2004, 3 (02) : 163 - 169
12345