A C-HCR assembly of branched DNA nanostructures for amplified uracil-DNA glycosylase assays

被引：38
|
作者：
Wang, Jing [1 ]
Pan, Min [1 ]
Wei, Jie [1 ]
Liu, Xiaoqing [1 ]
Wang, Fuan [1 ]
机构：
[1] Wuhan Univ, Coll Chem & Mol Sci, Minist Educ, Key Lab Analyt Chem Biol & Med, Wuhan 430072, Hubei, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2017年 / 53卷 / 96期
基金：
中国国家自然科学基金;
关键词：
HYBRIDIZATION CHAIN-REACTION; ULTRASENSITIVE DETECTION; AMPLIFICATION STRATEGY; SPATIAL-ORGANIZATION; SUBSTRATE; CIRCUITS; COMPLEX; SIGNAL; CELLS;
D O I：
10.1039/c7cc07057h
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
An autonomous nonenzymatic DNA machine has been successfully engineered based on a two-layered cascaded hybridization chain reaction (C-HCR) circuit, in which the tandem outputs of the upstream HCR-1 unit activate the downstream HCR-2 unit to induce successive repeated hybridizations, generating branched DNA structures and enabling sensitive and selective detection of uracil-DNA glycosylase and its inhibitors.
引用
收藏
页码：12878 / 12881
页数：4
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