Electric pulse-induced precipitation of biological macromolecules in electroporation

被引：30

作者：

Stapulionis, R ^{[1
]}

机构：

[1] Kaunas Med Univ, Inst Biomed Res, Kaunas, Lithuania

[2] Kaunas Med Univ, Dept Biochem, Kaunas, Lithuania

来源：

BIOELECTROCHEMISTRY AND BIOENERGETICS | 1999年 / 48卷 / 01期

关键词：

electroporation method; pulse-induced precipitation; biological macromolecules; artefact; solubilization of electrode;

D O I：

10.1016/S0302-4598(98)00206-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We found that electric discharge through solution of biological macromolecules (DNA, RNA and proteins) causes precipitation of significant portions of these macromolecules. This precipitation is a consequence of the interaction of biological macromolecules with the metal ions solubilized from the anode plate by the electric pulse, and occurs in both absence and presence of the cells in poration medium. Precipitated fractions of macromolecules sediments at the centrifugation speed used to pellet eukaryotic cells and does not dissolve when washed with buffer. Our data indicate a complication of the direct evaluation of electroporation efficiency based on the assumption that electroporated biological macromolecules which remain associated with the cells after several washes, are successfully electroinjected into the cytoplasm of cells. (C) 1999 Elsevier Science S.A. All rights reserved.

引用

页码：249 / 254

页数：6

共 18 条

[1] THE NUMBER OF MOLECULES TAKEN UP BY ELECTROPORATED CELLS - QUANTITATIVE-DETERMINATION [J].