Development of a Multiplex PCR Assay Targeting O-Antigen Modification Genes for Molecular Serotyping of Shigella flexneri

被引：44

作者：

Sun, Qiangzheng ^{[1
]}

Lan, Ruiting ^{[2
]}

Wang, Yiting ^{[1
]}

Zhao, Ailan ^{[1
]}

Zhang, Shaomin ^{[1
]}

Wang, Jianping ^{[1
]}

Wang, Yan ^{[1
]}

Xia, Shengli ^{[3
]}

Jin, Dong ^{[1
]}

Cui, Zhigang ^{[1
]}

Zhao, Hongqing ^{[1
]}

Li, Zhenjun ^{[1
]}

Ye, Changyun ^{[1
]}

Zhang, Shuxia ^{[1
]}

Jing, Huaiqi ^{[1
]}

Xu, Jianguo ^{[1
]}

机构：

[1] China CDC, State Key Lab Infect Dis Prevent & Control, Natl Inst Communicable Dis Control & Prevent, Beijing, Peoples R China

[2] Univ New S Wales, Sch Biotechnol & Biomol Sci, Sydney, NSW 2052, Australia

[3] Henan Ctr Dis Control & Prevent, Branch Enter Dis Control & Prevent, Inst Infect Dis Control & Prevent, Zhengzhou, Peoples R China

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2011年 / 49卷 / 11期

关键词：

CONVERSION; STRAINS; BURDEN; GENOME; 1A;

D O I：

10.1128/JCM.01259-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Shigella flexneri is the major Shigella species that causes diarrheal disease in developing countries. It is further subdivided into 15 serotypes based on O-antigen structure. Serotyping of S. flexneri is important for epidemiological purposes. In this study, we developed a multiplex PCR assay targeting the O-antigen synthesis gene wzx and the O-antigen modification genes gtrI, gtrIC, gtrII, oac, gtrIV, gtrV, and gtrX for molecular serotyping of S. flexneri. The multiplex PCR assay contained eight sets of specific PCRs in a single tube and can identify 14 of the 15 serotypes (the exception being serotype Xv) of S. flexneri recognized thus far. A nearly perfect concordance (97.8%) between multiplex PCR assay and slide agglutination was observed when 358 S. flexneri strains of various serotypes were analyzed, except that 8 strains were carrying additional cryptic and/or defective serotype-specific genes. The multiplex PCR assay provides a rapid and specific method for the serotype identification of S. flexneri.

引用

页码：3766 / 3770

页数：5

共 21 条

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