Development and validation of an reversed-phase liquid chromatographic method for analysis of spiramycin and related substances

被引：21

作者：

Chepkwony, HK ^{[1
]}

Vermaelen, A ^{[1
]}

Roets, E ^{[1
]}

Hoogmartens, J ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Fac Farm Wetenschappen, Lab Farmaceut Chem & Anal Geneesmiddelen, B-3000 Louvain, Belgium

来源：

CHROMATOGRAPHIA | 2001年 / 54卷 / 1-2期

关键词：

column liquid chromatography; spiramycin; factorial design; robustness;

D O I：

10.1007/BF02491832

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple, robust, sensitive isocratic liquid chromatographic (LC) method suitable for the analysis of spiramycin is described. This method utilizes XTerra RP18 as the stationary phase and acetonitrile-0.2 M K2HPO4 (pH 6.5)-water, 39.5:5:55.5 (v/v), as mobile phase. The mobile phase flow rate is 1.0 mL min(-1); the column is maintained at 70 degreesC by immersion in a water-bath; and UV detection is performed at 232 nm. The method has good selectivity towards the major components (spiramycins I, II, and III), other related substances, and many impurities. The robustness of the method was evaluated by means of full-factorial experimental design.

引用

页码：51 / 56

页数：6

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