Anti-inflammatory and anti-arthritic properties of naringenin via attenuation of NF-κB and activation of the heme oxygenase (HO)-1/related factor 2 pathway

被引:38
作者
Fan, Rong [1 ]
Pan, Tong [2 ]
Zhu, An-Li [3 ]
Zhang, Mei-Hong [4 ]
机构
[1] Liaocheng Peoples Hosp, Dept Rheumatism & Immunol, Liaocheng City, Shandong, Peoples R China
[2] Second Peoples Hosp Liaocheng, Dept Rheumatism & Immunol, Liaocheng City, Shandong, Peoples R China
[3] Liaocheng Peoples Hosp, Dept Tradit Chinese Med, Liaocheng City, Shandong, Peoples R China
[4] Second Peoples Hosp Liaocheng, Dept Orthopaed, Dept Urol, Liaocheng City, Shandong, Peoples R China
关键词
Naringenin; Complete Freund's adjuvant; NF-kappa B; Nrf-2/HO-1; pathway; INDUCED ARTHRITIS; WISTAR RATS; METHOTREXATE; INFLAMMATION; SUPPRESSES; LINN;
D O I
10.1016/j.pharep.2017.03.020
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Background: Naringenin, a bioflavonoid present in various species of citrus fruit, tomatoes and grapes, has been shown to have various pharmacological effects. We evaluated the anti-arthritic potential of naringenin in formaldehyde-induced inflammation and complete Freund's adjuvant (CFA)-induced arthritis. Methods: For both evaluations, rats were divided into groups of six. Different doses of naringenin (5, 10 and 20 mg/kg) were used in the models. Body weight and the arthritic index were assessed. Biochemical and antioxidant parameters were determined. Naringenin dose-dependently reduced joint inflammation, decreasing the joint diameter and inflammatory cell infiltration. Results: Naringenin-treated rats showed an improvement in the synovium redox status (down-regulation of malondialdehyde and glutathione and up-regulation of Catalase (CAT) and superoxide dismutase levels). Naringenin significantly reduced the level of the inflammatory marker TNF-alpha. Naringenin increased Nrf-2/HO-1 and reduced NF-kappa B mRNA levels in CFA-treated animal joints. Additionally, naringenin treatment decreased the expression of extracellular matrix degrading enzymes, such as matrix metalloproteinase (MMP-3 and MMP-9), in CFA-induced arthritic rats. Conclusions: We conclude that naringenin exerts anti-arthritic effects by downregulating NF-kappa B and activating the Nrf-2/HO-1 pathway. (c) 2017 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier Sp. z o.o. All rights reserved.
引用
收藏
页码:1021 / 1029
页数:9
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