MicroRNAs (miRNAs) function as crucial suppressors of gene expression via translational repression or direct mRNA degradation. However, the mechanism of multi-gene regulation by a host miRNA in antiviral immunity has not been extensively explored. In this study, the regulation of two white spot syndrome virus (WSSV) genes by its host (Marsupenaeus japonicus shrimp) miRNA (shrimp miR-1000) was characterized. The miRNA target gene prediction showed that only two virus genes (wsv191 and wsv407) might be the targets of miR-1000. The results of insect cell transfection assays revealed that shrimp miR-1000 could target multiple virus genes (wsv191 and wsv407). The mRNA degradation analysis and RNA FISH (fluorescence in situ hybridization) analysis indicated that miR-1000 triggered the mRNA degradation of target genes through 5'-3'exonucleolytic digestion in vivo and thereby inhibited the virus infection in shrimp. The miRNA-mediated 5'-3'exonucleolytic digestion of targetmRNAs stopped near the 3'UTR (3'untranslated region) sequence complementary to the seed sequence of miR-1000. Therefore, our study provided novel insights into how a host miRNA targeted multiple viral genes and prevented host from virus infection.
机构:
UCL Royal Free & Univ Coll, Sch Med, Ctr Hepatol, London, England
Univ New S Wales, St Vincents Clin Sch, Sydney, NSW, AustraliaUCL Royal Free & Univ Coll, Sch Med, Ctr Hepatol, London, England
Danta, M.
;
Semmo, N.
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机构:
Nuffield Dept Clin Med, Oxford, England
Univ Freiburg, Dept Med 2, Freiburg, GermanyUCL Royal Free & Univ Coll, Sch Med, Ctr Hepatol, London, England
Semmo, N.
;
Fabris, P.
论文数: 0引用数: 0
h-index: 0
机构:
Nuffield Dept Clin Med, Oxford, England
San Bortolo Hosp, Dept Infect Dis & Trop Med, Vicenza, ItalyUCL Royal Free & Univ Coll, Sch Med, Ctr Hepatol, London, England
Fabris, P.
;
Brown, D.
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h-index: 0
机构:
UCL Royal Free & Univ Coll, Sch Med, Ctr Hepatol, London, EnglandUCL Royal Free & Univ Coll, Sch Med, Ctr Hepatol, London, England
Brown, D.
;
Pybus, O. G.
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h-index: 0
机构:
Univ Oxford, Dept Zool, Oxford OX1 3PS, EnglandUCL Royal Free & Univ Coll, Sch Med, Ctr Hepatol, London, England
Pybus, O. G.
;
Sabin, C. A.
论文数: 0引用数: 0
h-index: 0
机构:
UCL Royal Free & Univ Coll, Sch Med, Dept Primary Care & Populat Sci, London, EnglandUCL Royal Free & Univ Coll, Sch Med, Ctr Hepatol, London, England
Sabin, C. A.
;
Bhagani, S.
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h-index: 0
机构:
UCL Royal Free & Univ Coll, Sch Med, Dept HIV Med, London, EnglandUCL Royal Free & Univ Coll, Sch Med, Ctr Hepatol, London, England
Bhagani, S.
;
Emery, V. C.
论文数: 0引用数: 0
h-index: 0
机构:
UCL Royal Free & Univ Coll, Sch Med, Dept Infect, London, EnglandUCL Royal Free & Univ Coll, Sch Med, Ctr Hepatol, London, England
Emery, V. C.
;
Dusheiko, G. M.
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h-index: 0
机构:
UCL Royal Free & Univ Coll, Sch Med, Ctr Hepatol, London, EnglandUCL Royal Free & Univ Coll, Sch Med, Ctr Hepatol, London, England
Dusheiko, G. M.
;
Klenerman, P.
论文数: 0引用数: 0
h-index: 0
机构:
Nuffield Dept Clin Med, Oxford, England
John Radcliffe Hosp, Biomed Res Ctr, Oxford OX3 9DU, EnglandUCL Royal Free & Univ Coll, Sch Med, Ctr Hepatol, London, England
机构:
Univ Wisconsin, Sch Med & Publ Hlth, Dept Dermatol, Madison, WI 53706 USAUniv Wisconsin, Sch Med & Publ Hlth, Dept Dermatol, Madison, WI 53706 USA
Elcheva, Irina
;
Goswami, Srikanta
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h-index: 0
机构:
Univ Wisconsin, Sch Med & Publ Hlth, Dept Dermatol, Madison, WI 53706 USAUniv Wisconsin, Sch Med & Publ Hlth, Dept Dermatol, Madison, WI 53706 USA
Goswami, Srikanta
;
Noubissi, Felicite K.
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h-index: 0
机构:
Univ Wisconsin, Sch Med & Publ Hlth, Dept Dermatol, Madison, WI 53706 USAUniv Wisconsin, Sch Med & Publ Hlth, Dept Dermatol, Madison, WI 53706 USA
Noubissi, Felicite K.
;
Spiegelman, Vladimir S.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Wisconsin, Sch Med & Publ Hlth, Dept Dermatol, Madison, WI 53706 USA
Univ Wisconsin, Sch Med & Publ Hlth, Paul P Carbone Comprehens Canc Ctr, Madison, WI 53706 USAUniv Wisconsin, Sch Med & Publ Hlth, Dept Dermatol, Madison, WI 53706 USA
机构:
UCL Royal Free & Univ Coll, Sch Med, Ctr Hepatol, London, England
Univ New S Wales, St Vincents Clin Sch, Sydney, NSW, AustraliaUCL Royal Free & Univ Coll, Sch Med, Ctr Hepatol, London, England
Danta, M.
;
Semmo, N.
论文数: 0引用数: 0
h-index: 0
机构:
Nuffield Dept Clin Med, Oxford, England
Univ Freiburg, Dept Med 2, Freiburg, GermanyUCL Royal Free & Univ Coll, Sch Med, Ctr Hepatol, London, England
Semmo, N.
;
Fabris, P.
论文数: 0引用数: 0
h-index: 0
机构:
Nuffield Dept Clin Med, Oxford, England
San Bortolo Hosp, Dept Infect Dis & Trop Med, Vicenza, ItalyUCL Royal Free & Univ Coll, Sch Med, Ctr Hepatol, London, England
Fabris, P.
;
Brown, D.
论文数: 0引用数: 0
h-index: 0
机构:
UCL Royal Free & Univ Coll, Sch Med, Ctr Hepatol, London, EnglandUCL Royal Free & Univ Coll, Sch Med, Ctr Hepatol, London, England
Brown, D.
;
Pybus, O. G.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Oxford, Dept Zool, Oxford OX1 3PS, EnglandUCL Royal Free & Univ Coll, Sch Med, Ctr Hepatol, London, England
Pybus, O. G.
;
Sabin, C. A.
论文数: 0引用数: 0
h-index: 0
机构:
UCL Royal Free & Univ Coll, Sch Med, Dept Primary Care & Populat Sci, London, EnglandUCL Royal Free & Univ Coll, Sch Med, Ctr Hepatol, London, England
Sabin, C. A.
;
Bhagani, S.
论文数: 0引用数: 0
h-index: 0
机构:
UCL Royal Free & Univ Coll, Sch Med, Dept HIV Med, London, EnglandUCL Royal Free & Univ Coll, Sch Med, Ctr Hepatol, London, England
Bhagani, S.
;
Emery, V. C.
论文数: 0引用数: 0
h-index: 0
机构:
UCL Royal Free & Univ Coll, Sch Med, Dept Infect, London, EnglandUCL Royal Free & Univ Coll, Sch Med, Ctr Hepatol, London, England
Emery, V. C.
;
Dusheiko, G. M.
论文数: 0引用数: 0
h-index: 0
机构:
UCL Royal Free & Univ Coll, Sch Med, Ctr Hepatol, London, EnglandUCL Royal Free & Univ Coll, Sch Med, Ctr Hepatol, London, England
Dusheiko, G. M.
;
Klenerman, P.
论文数: 0引用数: 0
h-index: 0
机构:
Nuffield Dept Clin Med, Oxford, England
John Radcliffe Hosp, Biomed Res Ctr, Oxford OX3 9DU, EnglandUCL Royal Free & Univ Coll, Sch Med, Ctr Hepatol, London, England
机构:
Univ Wisconsin, Sch Med & Publ Hlth, Dept Dermatol, Madison, WI 53706 USAUniv Wisconsin, Sch Med & Publ Hlth, Dept Dermatol, Madison, WI 53706 USA
Elcheva, Irina
;
Goswami, Srikanta
论文数: 0引用数: 0
h-index: 0
机构:
Univ Wisconsin, Sch Med & Publ Hlth, Dept Dermatol, Madison, WI 53706 USAUniv Wisconsin, Sch Med & Publ Hlth, Dept Dermatol, Madison, WI 53706 USA
Goswami, Srikanta
;
Noubissi, Felicite K.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Wisconsin, Sch Med & Publ Hlth, Dept Dermatol, Madison, WI 53706 USAUniv Wisconsin, Sch Med & Publ Hlth, Dept Dermatol, Madison, WI 53706 USA
Noubissi, Felicite K.
;
Spiegelman, Vladimir S.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Wisconsin, Sch Med & Publ Hlth, Dept Dermatol, Madison, WI 53706 USA
Univ Wisconsin, Sch Med & Publ Hlth, Paul P Carbone Comprehens Canc Ctr, Madison, WI 53706 USAUniv Wisconsin, Sch Med & Publ Hlth, Dept Dermatol, Madison, WI 53706 USA