Shrimp miR-1000 Functions in Antiviral Immunity by Simultaneously Triggering the Degradation of Two Viral mRNAs

被引:16
作者
Gong, Yi [1 ]
Ju, Chenyu [1 ]
Zhang, Xiaobo [1 ]
机构
[1] Zhejiang Univ, Coll Life Sci, Lab Marine Biol & Biotechnol, Qingdao Natl Lab Marine Sci & Technol, Hangzhou, Zhejiang, Peoples R China
来源
FRONTIERS IN IMMUNOLOGY | 2018年 / 9卷
基金
中国国家自然科学基金;
关键词
shrimp miR-1000; white spot syndrome virus gene; mRNA degradation; virus-host interactions; wsv191 and wsv407; SYNDROME VIRUS WSSV; MICRORNA; MIRNAS; IDENTIFICATION; APOPTOSIS; UBIQUITINATION; REPLICATION; EXPRESSION; NUCLEASE; PROTECTS;
D O I
10.3389/fimmu.2018.02999
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
MicroRNAs (miRNAs) function as crucial suppressors of gene expression via translational repression or direct mRNA degradation. However, the mechanism of multi-gene regulation by a host miRNA in antiviral immunity has not been extensively explored. In this study, the regulation of two white spot syndrome virus (WSSV) genes by its host (Marsupenaeus japonicus shrimp) miRNA (shrimp miR-1000) was characterized. The miRNA target gene prediction showed that only two virus genes (wsv191 and wsv407) might be the targets of miR-1000. The results of insect cell transfection assays revealed that shrimp miR-1000 could target multiple virus genes (wsv191 and wsv407). The mRNA degradation analysis and RNA FISH (fluorescence in situ hybridization) analysis indicated that miR-1000 triggered the mRNA degradation of target genes through 5'-3'exonucleolytic digestion in vivo and thereby inhibited the virus infection in shrimp. The miRNA-mediated 5'-3'exonucleolytic digestion of targetmRNAs stopped near the 3'UTR (3'untranslated region) sequence complementary to the seed sequence of miR-1000. Therefore, our study provided novel insights into how a host miRNA targeted multiple viral genes and prevented host from virus infection.
引用
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页数:14
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