Structure of a peptidoglycan-related polysaccharide from Providencia alcalifaciens O45

被引：9

作者：

Ovchinnikova, O. G. ^{[1
,2
]}

Liu, B. ^{[2
]}

Kocharova, N. A. ^{[1
]}

Shashkov, A. S. ^{[1
]}

Kondakova, A. N. ^{[1
]}

Siwinska, M. ^{[3
]}

Feng, L. ^{[2
,4
]}

Rozalski, A. ^{[5
]}

Wang, L. ^{[4
]}

Knirel, Y. A. ^{[1
]}

机构：

[1] Russian Acad Sci, Zelinsky Inst Organ Chem, Moscow 119991, Russia

[2] Nankai Univ, TEDA Sch Biol Sci & Biotechnol, TEDA, Tianjin 300457, Peoples R China

[3] Univ Lodz, Inst Microbiol Biotechnol & Immunol, Dept Gen Microbiol, PL-90237 Lodz, Poland

[4] Nankai Univ, TEDA, Tianjin Key Lab Microbial Funct Genom, Tianjin 300457, Peoples R China

[5] Univ Lodz, Inst Microbiol Biotechnol & Immunol, Dept Immunobiol Bacteria, PL-90237 Lodz, Poland

来源：

BIOCHEMISTRY-MOSCOW | 2012年 / 77卷 / 06期

基金：

俄罗斯基础研究基金会; 高等学校博士学科点专项科研基金; 中国国家自然科学基金;

关键词：

Providencia alcalifaciens; lipopolysaccharide; N-acetylmuramic acid; structure; bacterial polysaccharide; peptidoglycan; PSEUDOMONAS-AERUGINOSA; LIPOPOLYSACCHARIDE; LOCUS; ACID; GENE;

D O I：

10.1134/S0006297912060077

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A polysaccharide was isolated from the opportunistic human pathogen Providencia alcalifaciens O45:H26 by extraction with aqueous phenol and studied by sugar and methylation analyses along with H-1 and C-13 NMR spectroscopy, including two-dimensional ROESY and H-detected H-1,C-13 HSQC experiments. The polysaccharide contains N-acetylglu-cosamine and N-acetylmuramic acid (D-GlcpNAc3Rlac) amidated with L-alanine and has the following structure: <Equation ID="Equ1"> <MediaObject> </MediaObject> </Equation> The polysaccharide possesses a remarkable structural similarity to the bacterial cell wall peptidoglycan. It is not unique to the strain studied but is common to strains of at least four P. alcalifaciens O-serogroups (O3, O24, O38, and O45). No evidence was obtained that the polysaccharide is associated with the LPS, and hence it might represent a bacterial capsule component.

引用

页码：609 / 615

页数：7

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