Structure of a peptidoglycan-related polysaccharide from Providencia alcalifaciens O45

被引：9

作者：

Ovchinnikova, O. G. ^{[1
,2
]}

Liu, B. ^{[2
]}

Kocharova, N. A. ^{[1
]}

Shashkov, A. S. ^{[1
]}

Kondakova, A. N. ^{[1
]}

Siwinska, M. ^{[3
]}

Feng, L. ^{[2
,4
]}

Rozalski, A. ^{[5
]}

Wang, L. ^{[4
]}

Knirel, Y. A. ^{[1
]}

机构：

[1] Russian Acad Sci, Zelinsky Inst Organ Chem, Moscow 119991, Russia

[2] Nankai Univ, TEDA Sch Biol Sci & Biotechnol, TEDA, Tianjin 300457, Peoples R China

[3] Univ Lodz, Inst Microbiol Biotechnol & Immunol, Dept Gen Microbiol, PL-90237 Lodz, Poland

[4] Nankai Univ, TEDA, Tianjin Key Lab Microbial Funct Genom, Tianjin 300457, Peoples R China

[5] Univ Lodz, Inst Microbiol Biotechnol & Immunol, Dept Immunobiol Bacteria, PL-90237 Lodz, Poland

来源：

BIOCHEMISTRY-MOSCOW | 2012年 / 77卷 / 06期

基金：

高等学校博士学科点专项科研基金; 中国国家自然科学基金; 俄罗斯基础研究基金会;

关键词：

Providencia alcalifaciens; lipopolysaccharide; N-acetylmuramic acid; structure; bacterial polysaccharide; peptidoglycan; PSEUDOMONAS-AERUGINOSA; LIPOPOLYSACCHARIDE; LOCUS; ACID; GENE;

D O I：

10.1134/S0006297912060077

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A polysaccharide was isolated from the opportunistic human pathogen Providencia alcalifaciens O45:H26 by extraction with aqueous phenol and studied by sugar and methylation analyses along with H-1 and C-13 NMR spectroscopy, including two-dimensional ROESY and H-detected H-1,C-13 HSQC experiments. The polysaccharide contains N-acetylglu-cosamine and N-acetylmuramic acid (D-GlcpNAc3Rlac) amidated with L-alanine and has the following structure: <Equation ID="Equ1"> <MediaObject> </MediaObject> </Equation> The polysaccharide possesses a remarkable structural similarity to the bacterial cell wall peptidoglycan. It is not unique to the strain studied but is common to strains of at least four P. alcalifaciens O-serogroups (O3, O24, O38, and O45). No evidence was obtained that the polysaccharide is associated with the LPS, and hence it might represent a bacterial capsule component.

引用

页码：609 / 615

页数：7

共 25 条

[1] LIPOPOLYSACCHARIDE OF PROVIDENCIA-RETTGERI - CHEMICAL STUDIES AND TAXONOMICAL IMPLICATIONS [J].

BASU, S ;

RADZIEJEWSKALEBRECHT, J ;

MAYER, H .

ARCHIVES OF MICROBIOLOGY, 1986, 144 (03) :213-218

[2] C-13 NUCLEAR MAGNETIC-RESONANCE SPECTROSCOPY OF MONOSACCHARIDES [J].

BOCK, K ;

PEDERSEN, C .

ADVANCES IN CARBOHYDRATE CHEMISTRY AND BIOCHEMISTRY, 1983, 41 :27-66

[3]

Dean CR, 2000, FEMS MICROBIOL LETT, V187, P59, DOI 10.1111/j.1574-6968.2000.tb09137.x

[4]

Ewing W.H., 1986, IDENTIFICATION ENTER

[5] THE PROVIDENCE GROUP - AN INTERMEDIATE GROUP OF ENTERIC BACTERIA [J].

EWING, WH ;

TANNER, KE ;

DENNARD, DA .

JOURNAL OF INFECTIOUS DISEASES, 1954, 94 (02) :134-140

[6]

HAKOMORI SJ, 1964, BIOCHEM TOKYO, V55, P205

[7]

Knirel YA, 2011, BACTERIAL LIPOPOLYSACCHARIDES: STRUCTURE, CHEMICAL SYNTHESIS, BIOGENESIS AND INTERACTION WITH HOST CELLS, P41, DOI 10.1007/978-3-7091-0733-1_3

[8] Conserved and variable structural features in the lipopolysaccharide of Pseudomonas aeruginosa [J].

Knirel, Yuriy A. ;

Bystrova, Olga V. ;

Kocharova, Nina A. ;

Zaehringer, Ulrich ;

Pier, Gerald B. .

JOURNAL OF ENDOTOXIN RESEARCH, 2006, 12 (06) :324-336

[9] Structure of the O-specific polysaccharide of Providencia alcalifaciens O16 containing N-acetylmuramic acid [J].

Kocharova, NA ;

Zatonsky, GV ;

Bystrova, OV ;

Ziolkowski, A ;

Wykrota, M ;

Shashkov, AS ;

Knirel, YA ;

Rozalski, A .

CARBOHYDRATE RESEARCH, 2002, 337 (18) :1667-1671

[10] Mass-spectrometric studies of Providencia SR-Form lipopolysaccharides and elucidation of the biological repeating unit structure of Providencia rustigianii O14-polysaccharide [J].

Kondakova, Anna N. ;

Vinogradov, Evgeny ;

Lindner, Buko ;

Kocharova, Nina A. ;

Rozalski, Antoni ;

Knirel, Yuriy A. .

JOURNAL OF CARBOHYDRATE CHEMISTRY, 2007, 26 (7-9) :497-512

← 1 2 3 →