Study on Ultrasonic and Microwave Assisted Extraction of Flavonoids from Clematis Huchouensis Tamura

The paper selects the dry medical materials of Clematis huchouensis Tamura as the material, and researches the extracted technical parameters of flavonoid including liquid material proportion, microwave power, extraction time and extraction temperature. The single-factor experiment indicates that the liquid material proportion is 20: 1, the microwave power is 120w, the extraction time is 30s and the temperature is 30 degrees C, which has good extraction effect. The orthogonal design experiment indicates that the best extraction process is liquid-material ratio is 15: 1, the microwave power is 160W, the time is 35s, the temperature is 35 degrees C, and the extraction rate reaches 0.706mg/g. Clematis Huchouensis Tamura is also called Gold Scissors and Ranunculaceae Clematis Huchouensis, and it is the plant of Ranunculaceae clematis. It mainly grows in Huzhou of Zhejiang. It has the efficacy of anticancer, expelling wind-damp, detoxification and decreasing swelling and relieving pain. Using external application or decoction of medicinal ingredients to treat rheumatic arthritis, deep abscesses and brain tumor has a long history in folk [1, 2], and the efficacy is evident. But there is no agreement on effective materials especially anti-cancer activity components. The early research results indicate that cancer-inhibition active substances relate to flavonoid especially kaempferol. The methods of extracting flavonoid of Clematis Huchouensis Tamura include Soxhlet extraction [3], ultrasound assisted extraction [4] and ultrasonic-assisted extraction [5]. The paper selects alcohol as solvent, uses the ultrasound and ultrasonic-assisted collaboration method to extract flavonoid of Clematis Huchouensis Tamura, and observes the influence of liquid-material ratio, power, extraction time and temperature on extraction effect.