Direct Detection of Equine Herpesvirus Type 1 DNA in Nasal Swabs by Loop-Mediated Isothermal Amplification (LAMP)

被引：15
作者：
Nemoto, Manabu [1 ]
Ohta, Minoru [2 ]
Tsujimura, Koji [1 ]
Bannai, Hiroshi [1 ]
Yamanaka, Takashi [1 ]
Kondo, Takashi [1 ]
Matsumura, Tomio [1 ]
机构：
[1] Japan Racing Assoc, Equine Res Inst, Epizoot Res Ctr, Shimotsuke, Tochigi 3290412, Japan
[2] Japan Racing Assoc, Ritto Training Ctr, Racehorse Clin, Shiga 5203085, Japan
来源：
JOURNAL OF VETERINARY MEDICAL SCIENCE | 2011年 / 73卷 / 09期
关键词：
equine herpesvirus type 1 (EHV-1); heat treatment; loop-mediated isothermal amplification (LAMP); PRIMERS; SERUM;
D O I：
10.1292/jvms.11-0065
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
We evaluated loop-mediated isothermal amplification (LAMP) as a means of detecting equine herpesvirus type 1 (EHV-1) DNA directly from nasal swabs. To increase the sensitivity, we added a step in which the samples were heat-treated to the original LAMP procedure. The detection limit of the LAMP assay with heat treatment was 10 times more sensitive than the original LAMP assay even when the DNA extraction step was omitted. In addition, the LAMP assay with heat treatment was more sensitive than the original LAMP assay and the polymerase chain reaction using clinical samples. The LAMP assay with heat treatment is easy to perform and so should be applicable to the diagnosis of EHV-1 infections in clinical laboratories.
引用
收藏
页码：1225 / 1227
页数：3
相关论文
共 17 条
[1]  
Allen G. P., 2004, Infectious diseases of livestock. Volume Two, P829
[2]  
Brosnahan MM, 2009, INFECT DIS HORSE, P41
[3]   Rapid diagnosis of herpes simplex virus infection by a loop-mediated isothermal amplification method [J].
Enomoto, Y ;
Yoshikawa, T ;
Ihira, M ;
Akimoto, S ;
Miyake, F ;
Usui, C ;
Suga, S ;
Suzuki, K ;
Kawana, T ;
Nishiyama, Y ;
Asano, Y .
JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (02) :951-955
[4]   Direct detection of human herpesvirus 6 DNA in serum by the loop-mediated isothermal amplification method [J].
Ihira, Masaru ;
Akimoto, Shiho ;
Miyake, Fumi ;
Fujita, Ayano ;
Sugata, Ken ;
Suga, Sadao ;
Ohashi, Masahiro ;
Nishimura, Naoko ;
Ozaki, Takao ;
Asano, Yoshizo ;
Yoshikawa, Tetsushi .
JOURNAL OF CLINICAL VIROLOGY, 2007, 39 (01) :22-26
[5]   Direct detection of human herpesvirus 6 DNA in serum by variant specific loop-mediated isothermal amplification in hematopoietic stem cell transplant recipients [J].
Ihira, Masaru ;
Sugiyama, Hiroko ;
Enomoto, Yoshihiko ;
Higashimoto, Yuki ;
Sugata, Ken ;
Asano, Yoshizo ;
Yoshikawa, Tetsushi .
JOURNAL OF VIROLOGICAL METHODS, 2010, 167 (01) :103-106
[6]   Tolerance of loop-mediated isothermal amplification to a culture medium and biological substances [J].
Kaneko, Hisatoshi ;
Kawana, Takashi ;
Fukushima, Eiko ;
Suzutani, Tatsuo .
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 2007, 70 (03) :499-501
[7]   RAPID, SINGLE-STEP DIFFERENTIATION OF EQUID HERPESVIRUS-1 AND HERPESVIRUS-4 FROM CLINICAL MATERIAL USING THE POLYMERASE CHAIN-REACTION AND VIRUS-SPECIFIC PRIMERS [J].
LAWRENCE, GL ;
GILKERSON, J ;
LOVE, DN ;
SABINE, M ;
WHALLEY, JM .
JOURNAL OF VIROLOGICAL METHODS, 1994, 47 (1-2) :59-72
[8]   An equine herpesvirus type 1 recombinant with a deletion in the gE and gI genes is avirulent in young horses [J].
Matsumura, T ;
Kondo, T ;
Sugita, S ;
Damiani, AM ;
O'Callaghan, DJ ;
Imagawa, H .
VIROLOGY, 1998, 242 (01) :68-79
[9]   EPIZOOTIOLOGICAL ASPECTS OF TYPE-1 AND TYPE-4 EQUINE HERPESVIRUS INFECTIONS AMONG HORSE POPULATIONS [J].
MATSUMURA, T ;
SUGIURA, T ;
IMAGAWA, H ;
FUKUNAGA, Y ;
KAMADA, M .
JOURNAL OF VETERINARY MEDICAL SCIENCE, 1992, 54 (02) :207-211
[10]   Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation [J].
Mori, Y ;
Nagamine, K ;
Tomita, N ;
Notomi, T .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 289 (01) :150-154
12